Imperial College London
Alternate

DrNesrinaImami

Faculty of Medicine, Department of Infectious Disease

Reader in Immunology
 
 
 
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Contact

 

+44 (0)20 3315 5987n.imami Website

 
 
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Location

 

I.2.8Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cocker:2020:10.3389/fimmu.2020.00153,
author = {Cocker, ATH and Shah, NM and Raj, I and Dermont, S and Khan, W and Mandalia, S and Imami, N and Johnson, MR},
doi = {10.3389/fimmu.2020.00153},
journal = {Front Immunol},
title = {Pregnancy Gestation Impacts on HIV-1-Specific Granzyme B Response and Central Memory CD4 T Cells.},
url = {http://dx.doi.org/10.3389/fimmu.2020.00153},
volume = {11},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Pregnancy induces alterations in peripheral T-cell populations with both changes in subset frequencies and anti-viral responses found to alter with gestation. In HIV-1 positive women anti-HIV-1 responses are associated with transmission risk, however detailed investigation into both HIV-1-specific memory responses associated with HIV-1 control and T-cell subset changes during pregnancy have not been undertaken. In this study we aimed to define pregnancy and gestation related changes to HIV-1-specific responses and T-cell phenotype in ART treated HIV-1 positive pregnant women. Eleven non-pregnant and 24 pregnant HIV-1 positive women were recruited, peripheral blood samples taken, fresh cells isolated, and compared using ELISpot assays and flow cytometry analysis. Clinical data were collected as part of standard care, and non-parametric statistics used. Alterations in induced IFNγ, IL-2, IL-10, and granzyme B secretion by peripheral blood mononuclear cells in response to HIV-1 Gag and Nef peptide pools and changes in T-cell subsets between pregnant and non-pregnant women were assessed, with data correlated with participant clinical parameters and longitudinal analysis performed. Cross-sectional comparison identified decreased IL-10 Nef response in HIV-1 positive pregnant women compared to non-pregnant, while correlations exhibited reversed Gag and Nef cytokine and protease response associations between groups. Longitudinal analysis of pregnant participants demonstrated transient increases in Gag granzyme B response and in the central memory CD4 T-cell subset frequency during their second trimester, with a decrease in CD4 effector memory T cells from their second to third trimester. Gag and Nef HIV-1-specific responses diverge with pregnancy time-point, coinciding with relevant T-cell phenotype, and gestation associated immunological adaptations. Decreased IL-10 Nef and both increased granzyme B Gag response and central memory CD4 T cells implies that amplified a
AU  - Cocker,ATH
AU  - Shah,NM
AU  - Raj,I
AU  - Dermont,S
AU  - Khan,W
AU  - Mandalia,S
AU  - Imami,N
AU  - Johnson,MR
DO  - 10.3389/fimmu.2020.00153
PY  - 2020///
TI  - Pregnancy Gestation Impacts on HIV-1-Specific Granzyme B Response and Central Memory CD4 T Cells.
T2  - Front Immunol
UR  - http://dx.doi.org/10.3389/fimmu.2020.00153
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32117291
UR  - http://hdl.handle.net/10044/1/77290
VL  - 11
ER  -
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