Imperial College London
Alternate

Professor Oscar Ces

Faculty of Natural Sciences, Department of Chemistry

Head of Department, August von Hofmann Chair of Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 3754o.ces Website

 
 
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Location

 

G04AMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Chatzimichail:2018:10.1038/s41598-018-32706-6,
author = {Chatzimichail, S and Supramaniam, P and Ces, O and Salehi-Reyhani, S},
doi = {10.1038/s41598-018-32706-6},
journal = {Scientific Reports},
title = {Micropatterning of planar metal electrodes by vacuum filling microfluidic channel geometries},
url = {http://dx.doi.org/10.1038/s41598-018-32706-6},
volume = {8},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - We present a simple, facile method to micropattern planar metal electrodes defined by the geometry of a microfluidic channel network template. By introducing aqueous solutions of metal into reversibly adhered PDMS devices by desiccation instead of flow, we are able to produce difficult to pattern “dead end” or discontinuous features with ease. We characterize electrodes fabricated using this method and perform electrical lysis of mammalian cancer cells and demonstrate their use as part of an antibody capture assay for GFP. Cell lysis in microwell arrays is achieved using the electrodes and the protein released is detected using an antibody microarray. We show how the template channels used as part of the workflow for patterning the electrodes may be produced using photolithography-free methods, such as laser micromachining and PDMS master moulding, and demonstrate how the use of an immiscible phase may be employed to create electrode spacings on the order of 25 – 50 μm, that overcome the current resolution limits of such methods. This work demonstrates how the rapid prototyping of electrodes for use in total analysis systems can be achieved on the bench with little or no need for centralized facilities.
AU  - Chatzimichail,S
AU  - Supramaniam,P
AU  - Ces,O
AU  - Salehi-Reyhani,S
DO  - 10.1038/s41598-018-32706-6
PY  - 2018///
SN  - 2045-2322
TI  - Micropatterning of planar metal electrodes by vacuum filling microfluidic channel geometries
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-018-32706-6
UR  - http://hdl.handle.net/10044/1/64755
VL  - 8
ER  -
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