Imperial College London
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ProfessorPeterLee

Faculty of Engineering, Department of Materials

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 6801p.d.lee

 
 
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Location

 

102Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bhartiya:2020:10.1107/S1600577519015583,
author = {Bhartiya, A and Madi, K and Disney, CM and Courtois, L and Jupe, A and Zhang, F and Bodey, AJ and Lee, P and Rau, C and Robinson, IK and Yusuf, M},
doi = {10.1107/S1600577519015583},
journal = {Journal of Synchrotron Radiation},
pages = {158--163},
title = {Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays},
url = {http://dx.doi.org/10.1107/S1600577519015583},
volume = {27},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Advanced imaging is useful for understanding the three-dimensional (3D) growth of cells. X-ray tomography serves as a powerful noninvasive, nondestructive technique that can fulfill these purposes by providing information about cell growth within 3D platforms. There are a limited number of studies taking advantage of synchrotron X-rays, which provides a large field of view and suitable resolution to image cells within specific biomaterials. In this study, X-ray synchrotron radiation microtomography at Diamond Light Source and advanced image processing were used to investigate cellular infiltration of HeLa cells within poly L-lactide (PLLA) scaffolds. This study demonstrates that synchrotron X-rays using phase contrast is a useful method to understand the 3D growth of cells in PLLA electrospun scaffolds. Two different fiber diameter (2 and 4µm) scaffolds with different pore sizes, grown over 2, 5 and 8 days in vitro, were examined for infiltration and cell connectivity. After performing visualization by segmentation of the cells from the fibers, the results clearly show deeper cell growth and higher cellular interconnectivity in the 4µm fiber diameter scaffold. This indicates the potential for using such 3D technology to study cell-scaffold interactions for future medical use.
AU  - Bhartiya,A
AU  - Madi,K
AU  - Disney,CM
AU  - Courtois,L
AU  - Jupe,A
AU  - Zhang,F
AU  - Bodey,AJ
AU  - Lee,P
AU  - Rau,C
AU  - Robinson,IK
AU  - Yusuf,M
DO  - 10.1107/S1600577519015583
EP  - 163
PY  - 2020///
SN  - 0909-0495
SP  - 158
TI  - Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays
T2  - Journal of Synchrotron Radiation
UR  - http://dx.doi.org/10.1107/S1600577519015583
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31868748
UR  - http://hdl.handle.net/10044/1/76225
VL  - 27
ER  -
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