Imperial College London

ProfessorPaulFreemont

Faculty of MedicineDepartment of Medicine

Chair in Protein Crystallography
 
 
 
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Contact

 

+44 (0)20 7594 5327p.freemont

 
 
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Location

 

259Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

246 results found

Newman RH, Freemont PS, 1997, Electron microscopy of thin protein crystals from vapour diffusion 'hanging drops' provides structural information at intermediate resolution, NATO Advanced Study Institute on Electron Crystallography, Publisher: SPRINGER, Pages: 405-406, ISSN: 0168-132X

CONFERENCE PAPER

Rothwell DG, Barzilay G, Gorman M, Morera S, Freemont P, Hickson IDet al., 1997, The structure and functions of the HAP1/Ref-1 protein, ONCOLOGY RESEARCH, Vol: 9, Pages: 275-280, ISSN: 0965-0407

JOURNAL ARTICLE

Vrielink A, Freemont PS, 1996, Chapter 5 Protein-nucleic acid recognition and interactions, Principles of Medical Biology, Vol: 5, Pages: 85-115, ISSN: 1569-2582

The application of protein crystallography and two-dimensional NMR to study proteins which interact with DNA has provided detailed information about protein-DNA recognition and specificity. Numerous structural motifs have evolved to allow a diverse variety of interactions between proteins and DNA. These include motifs such as the helix-turn-helix, zinc finger, basic region leucine zipper, and ribbon-helix-helix, some of which are found in both prokaryotes and eukaryotes. DNA recognition is usually achieved through specific protein amino acid-DNA base pair interactions which can be mediated via water molecules, in either the major or minor grooves of the DNA. However, interactions between protein side chains and the phosphodiester backbone is a common feature of non-sequence-specific protein-DNA recognition. The flexibility and existence of DNA in a number of different conformations contributes significantly to the overall process of protein-DNA recognition. The enormous variety of protein structures and motifs which can interact with DNA probably reflects the diversity of biological function which requires the formation of protein-DNA complexes. © 1996 Elsevier B.V. All rights reserved.

JOURNAL ARTICLE

Saurin A, Borden K, Boddy M, Freemont Pet al., 1996, Does this have a familiar RING? (vol 21, pg 208, 1996), TRENDS IN BIOCHEMICAL SCIENCES, Vol: 21, Pages: 453-453, ISSN: 0968-0004

JOURNAL ARTICLE

Newman RH, Freemont PS, 1996, Use of planar lipid monolayers for 2D crystallisation of proteins and simple analysis of specific lipid-peptide interactions, 7th International Conference on Organized Molecular Films (LB7), Publisher: ELSEVIER SCIENCE SA LAUSANNE, Pages: 18-23, ISSN: 0040-6090

CONFERENCE PAPER

Boddy MN, Howe K, Etkin LD, Solomon E, Freemont PSet al., 1996, PIC 1, a novel ubiquitin-like protein which interacts with the PML component of a multiprotein complex that is disrupted in acute promyelocytic leukaemia, ONCOGENE, Vol: 13, Pages: 971-982, ISSN: 0950-9232

JOURNAL ARTICLE

Borden KLB, Freemont PS, 1996, The RING finger domain: A recent example of a sequence-structure family, CURRENT OPINION IN STRUCTURAL BIOLOGY, Vol: 6, Pages: 395-401, ISSN: 0959-440X

JOURNAL ARTICLE

Saurin AJ, Borden KLB, Boddy MN, Freemont PSet al., 1996, Does this have a familiar RING?, TRENDS IN BIOCHEMICAL SCIENCES, Vol: 21, Pages: 208-214, ISSN: 0968-0004

JOURNAL ARTICLE

Newman RH, Whitehead P, Lally J, Coffer A, Freemont Pet al., 1996, 20S human proteasomes bind with a specific orientation to lipid monolayers in vitro, BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, Vol: 1281, Pages: 111-116, ISSN: 0005-2736

JOURNAL ARTICLE

Borden KLB, Lally JM, Martin SR, OReilly NJ, Solomon E, Freemont PSet al., 1996, In vivo and in vitro characterization of the B1 and B2 zinc-binding domains from the acute promyelocytic leukemia protooncoprotein PML, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 93, Pages: 1601-1606, ISSN: 0027-8424

JOURNAL ARTICLE

Otto WR, Rao JD, Cox HM, Kotzian E, Lee CY, Goodlad RA, Lane A, Gorman M, Freemont PA, Hansen HF, Pappin D, Wright NAet al., 1996, Effects of pancreatic spasmolytic polypeptide (PSP) on epithelial cell function, EUROPEAN JOURNAL OF BIOCHEMISTRY, Vol: 235, Pages: 64-72, ISSN: 0014-2956

JOURNAL ARTICLE

Brown DG, Freemont PS, 1996, Crystallography in the study of protein-DNA interactions., Methods Mol Biol, Vol: 56, Pages: 293-318, ISSN: 1064-3745

JOURNAL ARTICLE

BORDEN KLB, LALLY JM, MARTIN SR, OREILLY NJ, ETKIN LD, FREEMONT PSet al., 1995, NOVEL TOPOLOGY OF A ZINC-BINDING DOMAIN FROM A PROTEIN INVOLVED IN REGULATING EARLY XENOPUS DEVELOPMENT, EMBO JOURNAL, Vol: 14, Pages: 5947-5956, ISSN: 0261-4189

JOURNAL ARTICLE

Boddy MN, Howe K, Etkin LD, Solomon E, Freemont PSet al., 1995, PIC 1, a novel ubiquitin-like protein interacts with the PML component of a multiprotein complex that is disrupted in acute promyelocytic leukaemia., BLOOD, Vol: 86, Pages: 644-644, ISSN: 0006-4971

JOURNAL ARTICLE

Borden KLB, Boddy MN, Lally JL, Solomon E, Freemont Pet al., 1995, Studies of the molecular functions of the zinc binding domains in the acute promyelocytic leukemia proto oncoprotein, PML, BLOOD, Vol: 86, Pages: 1038-1038, ISSN: 0006-4971

JOURNAL ARTICLE

BORDEN KLB, BODDY MN, LALLY J, OREILLY NJ, MARTIN S, HOWE K, SOLOMON E, FREEMONT PSet al., 1995, THE SOLUTION STRUCTURE OF THE RING FINGER DOMAIN FROM THE ACUTE PROMYELOCYTIC LEUKEMIA PROTO-ONCOPROTEIN PML, EMBO JOURNAL, Vol: 14, Pages: 1532-1541, ISSN: 0261-4189

JOURNAL ARTICLE

SECKL MJ, NEWMAN RH, FREEMONT PS, ROZENGURT Eet al., 1995, SUBSTANCE P-RELATED ANTAGONISTS INHIBIT VASOPRESSIN AND BOMBESIN BUT NOT 5'-3-O-(THIO)TRIPHOSPHATE-STIMULATED INOSITOL PHOSPHATE PRODUCTION IN SWISS 3T3 CELLS, JOURNAL OF CELLULAR PHYSIOLOGY, Vol: 163, Pages: 87-95, ISSN: 0021-9541

JOURNAL ARTICLE

CHINERY R, BATES PA, DE A, FREEMONT PSet al., 1995, CHARACTERIZATION OF THE SINGLE-COPY TREFOIL PEPTIDES INTESTINAL TREFOIL FACTOR AND PS2 AND THEIR ABILITY TO FORM COVALENT DIMERS, FEBS LETTERS, Vol: 357, Pages: 50-54, ISSN: 0014-5793

JOURNAL ARTICLE

VRIELINK A, RUGER W, DRIESSEN HPC, FREEMONT PSet al., 1994, CRYSTAL-STRUCTURE OF THE DNA MODIFYING ENZYME BETA-GLUCOSYLTRANSFERASE IN THE PRESENCE AND ABSENCE OF THE SUBSTRATE URIDINE DIPHOSPHOGLUCOSE, EMBO JOURNAL, Vol: 13, Pages: 3413-3422, ISSN: 0261-4189

JOURNAL ARTICLE

BODDY MN, FREEMONT PS, BORDEN KLB, 1994, THE P53-ASSOCIATED PROTEIN MDM2 CONTAINS A NEWLY CHARACTERIZED ZINC-BINDING DOMAIN CALLED THE RING FINGER, TRENDS IN BIOCHEMICAL SCIENCES, Vol: 19, Pages: 198-199, ISSN: 0968-0004

JOURNAL ARTICLE

Newman RH, Carpenter E, Freemont PS, Blundell TL, Parker PJet al., 1994, Microcrystals of the beta 1 isoenzyme of protein kinase C: an electron microscopic study., Biochem J, Vol: 298 ( Pt 2), Pages: 391-393, ISSN: 0264-6021

Microcrystals of protein kinase C beta 1 have been grown from solutions of poly(ethylene glycol). Image analysis of electron micrographs of the protein crystals, which diffracted to 5.0 nm, revealed p3 symmetry with a unit cell of about 10.3 nm x 10.3 nm. The electron stain-excluding densities showed a three-domain ring structure in projection, giving kidney bean-shaped molecules of about 7.0 nm x 4.5 nm diameter, packed as trimers. The implications of these observations for the function of the enzyme are discussed.

JOURNAL ARTICLE

NEWMAN RH, CARPENTER E, FREEMONT PS, BLUNDELL TL, PARKER PJet al., 1994, MICROCRYSTALS OF THE BETA(1) ISOENZYME OF PROTEIN-KINASE-C - AN ELECTRON-MICROSCOPIC STUDY, BIOCHEMICAL JOURNAL, Vol: 298, Pages: 391-393, ISSN: 0264-6021

JOURNAL ARTICLE

DE A, BROWN DG, GORMAN MA, CARR M, SANDERSON MR, FREEMONT PSet al., 1994, CRYSTAL-STRUCTURE OF A DISULFIDE-LINKED TREFOIL MOTIF FOUND IN A LARGE FAMILY OF PUTATIVE GROWTH-FACTORS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 91, Pages: 1084-1088, ISSN: 0027-8424

JOURNAL ARTICLE

BORDEN KLB, MARTIN SR, OREILLY NJ, LALLY JM, REDDY BA, ETKIN LD, FREEMONT PSet al., 1993, CHARACTERIZATION OF A NOVEL CYSTEINE/HISTIDINE-RICH METAL-BINDING DOMAIN FROM XENOPUS NUCLEAR FACTOR XNF7, FEBS LETTERS, Vol: 335, Pages: 255-260, ISSN: 0014-5793

JOURNAL ARTICLE

FREEMONT PS, 1993, THE RING FINGER - A NOVEL PROTEIN-SEQUENCE MOTIF RELATED TO THE ZINC-FINGER, ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol: 684, Pages: 174-192, ISSN: 0077-8923

JOURNAL ARTICLE

FREEMONT P, 1993, STRUCTURAL BIOLOGY - MAX IN A COMPLEX AFFAIR, NATURE, Vol: 363, Pages: 20-21, ISSN: 0028-0836

JOURNAL ARTICLE

LOVERING R, HANSON IM, BORDEN KLB, MARTIN S, OREILLY NJ, EVAN GI, RAHMAN D, PAPPIN DJC, TROWSDALE J, FREEMONT PSet al., 1993, IDENTIFICATION AND PRELIMINARY CHARACTERIZATION OF A PROTEIN MOTIF RELATED TO THE ZINC FINGER, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 90, Pages: 2112-2116, ISSN: 0027-8424

JOURNAL ARTICLE

CERUTTI P, HIRT B, NABHOLZ M, NIGG E, HANAWALT PC, HOEIJMAKERS JHJ, WEEDA G, TROELSTRA C, KOKEN MHM, VANDERSPEK PJ, BOOTSMA D, MAHER VM, CHEN RH, MCGREGOR WG, WANG YC, LUKASH L, MAH MCM, BOLDT J, MCCORMICK JJ, AGUILAR F, POURZAND C, HUSSAIN P, ALBERTINI RJ, NICKLAS JA, ONEILL JP, HARRIS CC, BENNETT WP, LEHMANN TA, FORRESTER K, FELLEYBOSCO E, GERWIN BI, HOLLSTEIN MC, RAUSCHER FJ, CURRAN T, XANTHOUDAKIS S, HERRLICH P, SACHSENMAIER C, GEBEL S, RADLERPOHL A, AUER HP, KRAMER M, RAHMSDORF HJ, AARONSON SA, MARSHALL CJ, ASHWORTH A, HOWE LR, LEEVERS SJ, PATERSON HF, THORNEYCROFT SG, GOMEZ N, NAKIELNY S, TRAVERSE S, COHEN P, AYER D, BLACKWOOD EM, KRETZNER L, TIETJE K, EISENMAN RN, DAYAN S, GALLANT P, KREK W, MARIDOR G, PETER M, MOSES HL, DAGNINO L, FREXESSTEED M, JOHNSON MD, NIGRO JM, PIERCE DF, SOLOMON E, BORROW J, FREEMONT P, GILLARD E, GODDARD A, HOWE K, BERNSTEIN A, ZHANG LQ, LEE J, BERNS Aet al., 1993, GENETIC-DAMAGE AND ESCAPE FROM PROLIFERATION CONTROL - CONFERENCE HELD AT EPALINGES LAUSANNE, SWITZERLAND, JANUARY 14-15, 1993, INTERNATIONAL JOURNAL OF CANCER, Vol: 53, Pages: 161-177, ISSN: 0020-7136

JOURNAL ARTICLE

Solomon E, Borrow J, Freemont P, Gillard E, Goddard A, Howe Ket al., 1993, Molecular analysis of the (15;17) translocation found in acute promyelocytic leukaemia, Pages: 174-175, ISSN: 0020-7136

CONFERENCE PAPER

FREEMONT PS, 1993, THE RING FINGER - A NOVEL PROTEIN-SEQUENCE MOTIF RELATED TO THE ZINC-FINGER, CONF ON ZINC-FINGER PROTEINS IN ONCOGENESIS : DNA-BINDING AND GENE REGULATION, Publisher: NEW YORK ACAD SCIENCES, Pages: 174-192, ISSN: 0077-8923

CONFERENCE PAPER

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