Imperial College London

ProfessorPaulFreemont

Faculty of MedicineDepartment of Medicine

Chair in Protein Crystallography
 
 
 
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Contact

 

+44 (0)20 7594 5327p.freemont

 
 
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Location

 

259Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@phdthesis{Lai:2017,
author = {Lai, H-E and Obled, AMC and Chee, SM and Morgan, R and Sharma, S and Moore, S and Polizzi, K and Goss, RJM and Freemont, P},
title = {A GenoChemetic strategy for derivatization of the violacein natural product scaffold},
year = {2017}
}

RIS format (EndNote, RefMan)

TY  - THES
AB - Integrating synthetic chemistry with synthetic biology allows rapid access to xenobiotic compounds which may provide improved therapeutic activity. By supplementing an Escherichia coli strain expressing the violacein biosynthesis pathway with eight tryptophan substrate analogues or tryptophan halogenase RebH in vivo , 68 new-to-nature analogues of violacein were generated. Furthermore, 20 new derivatives were generated from brominated analogues via Suzuki-Miyaura cross-coupling reaction directly using the crude extract without prior purification. Herein, we demonstrate a flexible and rapid approach to access diverse chemical space that can be applied to a wide range of natural product scaffolds.
AU - Lai,H-E
AU - Obled,AMC
AU - Chee,SM
AU - Morgan,R
AU - Sharma,S
AU - Moore,S
AU - Polizzi,K
AU - Goss,RJM
AU - Freemont,P
PY - 2017///
TI - A GenoChemetic strategy for derivatization of the violacein natural product scaffold
ER -