Imperial College London

ProfessorPaulFreemont

Faculty of MedicineDepartment of Medicine

Chair in Protein Crystallography
 
 
 
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Contact

 

+44 (0)20 7594 5327p.freemont

 
 
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Location

 

259Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@phdthesis{Lai:2017,
author = {Lai, H-E and Obled, AMC and Chee, SM and Morgan, RM and Sharma, SV and Moore, SJ and Polizzi, KM and Goss, RJM and Freemont, PS},
title = {A GenoChemetic strategy for derivatization of the violacein natural product scaffold},
year = {2017}
}

RIS format (EndNote, RefMan)

TY  - THES
AB - <jats:p>Integrating synthetic chemistry with synthetic biology allows rapid access to xenobiotic compounds which may provide improved therapeutic activity. By supplementing an <jats:italic>Escherichia coli</jats:italic> strain expressing the violacein biosynthesis pathway with eight tryptophan substrate analogues or tryptophan halogenase RebH <jats:italic>in vivo</jats:italic>, 68 new-to-nature analogues of violacein were generated. Furthermore, 20 new derivatives were generated from brominated analogues via Suzuki-Miyaura cross-coupling reaction directly using the crude extract without prior purification. Herein, we demonstrate a flexible and rapid approach to access diverse chemical space that can be applied to a wide range of natural product scaffolds.</jats:p>
AU - Lai,H-E
AU - Obled,AMC
AU - Chee,SM
AU - Morgan,RM
AU - Sharma,SV
AU - Moore,SJ
AU - Polizzi,KM
AU - Goss,RJM
AU - Freemont,PS
PY - 2017///
TI - A GenoChemetic strategy for derivatization of the violacein natural product scaffold
ER -