Imperial College London

ProfessorPaulFreemont

Faculty of MedicineDepartment of Medicine

Chair in Protein Crystallography
 
 
 
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Contact

 

+44 (0)20 7594 5327p.freemont

 
 
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Location

 

259Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Moore:2017:10.1002/biot.201600678,
author = {Moore, SJ and Lai, HE and Needham, H and Polizzi, KM and Freemont, PS},
doi = {10.1002/biot.201600678},
journal = {Biotechnology Journal},
title = {Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool},
url = {http://dx.doi.org/10.1002/biot.201600678},
volume = {12},
year = {2017}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Streptomyces venezuelae is a promising chassis in synthetic biology for fine chemical and secondary metabolite pathway engineering. The potential of S. venezuelae could be further realized by expanding its capability with the introduction of its own in vitro transcription-translation (TX-TL) system. TX-TL is a fast and expanding technology for bottom-up design of complex gene expression tools, biosensors and protein manufacturing. Herein, we introduce a S. venezuelae TX-TL platform by reporting a streamlined protocol for cell-extract preparation, demonstrating high-yield synthesis of a codon-optimized sfGFP reporter and the prototyping of a synthetic tetracycline-inducible promoter in S. venezuelae TX-TL based on the tetO-TetR repressor system. The aim of this system is to provide a host for the homologous production of exotic enzymes from Actinobacteria secondary metabolism in vitro. As an example, the authors demonstrate the soluble synthesis of a selection of enzymes (12-70 kDa) from the Streptomyces rimosus oxytetracycline pathway.
AU - Moore,SJ
AU - Lai,HE
AU - Needham,H
AU - Polizzi,KM
AU - Freemont,PS
DO - 10.1002/biot.201600678
PY - 2017///
SN - 1860-7314
TI - Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool
T2 - Biotechnology Journal
UR - http://dx.doi.org/10.1002/biot.201600678
UR - http://www.ncbi.nlm.nih.gov/pubmed/28139884
UR - http://hdl.handle.net/10044/1/44768
VL - 12
ER -