Imperial College London

ProfessorPaulFreemont

Faculty of MedicineDepartment of Medicine

Chair in Protein Crystallography
 
 
 
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Contact

 

+44 (0)20 7594 5327p.freemont

 
 
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Location

 

259Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Moore:2017:10.1042/BST20170011,
author = {Moore, SJ and macdonald, JT and freemont, PS},
doi = {10.1042/BST20170011},
journal = {Biochemical Society Transactions},
pages = {785--791},
title = {Cell-free synthetic biology for in vitro prototype engineering},
url = {http://dx.doi.org/10.1042/BST20170011},
volume = {45},
year = {2017}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Cell-free transcription–translation is an expandingfield in synthetic biology as a rapidprototyping platform for blueprinting the design of synthetic biological devices. Exemplarefforts include translation of prototype designs into medical test kits for on-site identifica-tion of viruses (Zika and Ebola), while gene circuit cascades can be tested, debuggedand re-designed within rapid turnover times. Coupled with mathematical modelling, thisdiscipline lends itself towards the precision engineering of new synthetic life. The nextstages of cell-free look set to unlock new microbial hosts that remain slow to engineerand unsuited to rapid iterative design cycles. It is hoped that the development of suchsystems will provide new tools to aid the transition from cell-free prototype designs tofunctioning synthetic genetic circuits and engineered natural product pathways in livingcells.
AU - Moore,SJ
AU - macdonald,JT
AU - freemont,PS
DO - 10.1042/BST20170011
EP - 791
PY - 2017///
SN - 1470-8752
SP - 785
TI - Cell-free synthetic biology for in vitro prototype engineering
T2 - Biochemical Society Transactions
UR - http://dx.doi.org/10.1042/BST20170011
UR - http://hdl.handle.net/10044/1/46179
VL - 45
ER -