Imperial College London

ProfessorPaulFreemont

Faculty of MedicineDepartment of Medicine

Chair in Protein Crystallography
 
 
 
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Contact

 

+44 (0)20 7594 5327p.freemont

 
 
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Location

 

259Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wen:2017:10.1021/acssynbio.7b00219,
author = {Wen, KY and Cameron, L and Chappell, J and Jensen, K and Bell, DJ and Kelwick, R and Kopniczky, M and Davies, JC and Filloux, A and Freemont, PS},
doi = {10.1021/acssynbio.7b00219},
journal = {ACS Synthetic Biology},
pages = {2293--2301},
title = {A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples.},
url = {http://dx.doi.org/10.1021/acssynbio.7b00219},
volume = {6},
year = {2017}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Synthetic biology designed cell-free biosensors are a promising new tool for the detection of clinically relevant biomarkers in infectious diseases. Here, we report that a modular DNA-encoded biosensor in cell-free protein expression systems can be used to measure a bacterial biomarker of Pseudomonas aeruginosa infection from human sputum samples. By optimizing the cell-free system and sample extraction, we demonstrate that the quorum sensing molecule 3-oxo-C12-HSL in sputum samples from cystic fibrosis lungs can be quantitatively measured at nanomolar levels using our cell-free biosensor system, and is comparable to LC-MS measurements of the same samples. This study further illustrates the potential of modular cell-free biosensors as rapid, low-cost detection assays that can inform clinical practice.
AU - Wen,KY
AU - Cameron,L
AU - Chappell,J
AU - Jensen,K
AU - Bell,DJ
AU - Kelwick,R
AU - Kopniczky,M
AU - Davies,JC
AU - Filloux,A
AU - Freemont,PS
DO - 10.1021/acssynbio.7b00219
EP - 2301
PY - 2017///
SN - 2161-5063
SP - 2293
TI - A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples.
T2 - ACS Synthetic Biology
UR - http://dx.doi.org/10.1021/acssynbio.7b00219
UR - http://hdl.handle.net/10044/1/53616
VL - 6
ER -