Imperial College London
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ProfessorPaulFreemont

Faculty of Medicine, Department of Infectious Disease

Chair in Protein Crystallography
 
 
 
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Contact

 

+44 (0)20 7594 5327p.freemont

 
 
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Location

 

259Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{de:2020:10.1111/mmi.14406,
author = {de, Martín Garrido N and Crone, MA and Ramlaul, K and Simpson, PA and Freemont, PS and Aylett, CHS},
doi = {10.1111/mmi.14406},
journal = {Molecular Microbiology},
pages = {143--152},
title = {Bacteriophage MS2 displays unreported capsid variability assembling T = 4 and mixed capsids},
url = {http://dx.doi.org/10.1111/mmi.14406},
volume = {113},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Bacteriophage MS2 is a positive-sense, single-stranded RNA virus encapsulated in an asymmetric T = 3 pseudo-icosahedral capsid. It infects Escherichia coli through the F-pilus, which it binds through a maturation protein incorporated into its capsid. Cryogenic electron microscopy has previously shown that its genome is highly ordered within virions, and that it regulates the assembly process of the capsid. In this study we have assembled recombinant MS2 capsids with non-genomic RNA containing the capsid incorporation sequence, and investigated the structures formed, revealing that T = 3, T = 4 and mixed capsids between these two triangulation numbers are generated, and resolving structures of T = 3 and T = 4 capsids to 4 Å and 6 Å respectively. We conclude that the basic MS2 capsid can form a mix of T = 3 and T = 4 structures, supporting a role for the ordered genome in favouring the formation of functional T = 3 virions.
AU  - de,Martín Garrido N
AU  - Crone,MA
AU  - Ramlaul,K
AU  - Simpson,PA
AU  - Freemont,PS
AU  - Aylett,CHS
DO  - 10.1111/mmi.14406
EP  - 152
PY  - 2020///
SN  - 0950-382X
SP  - 143
TI  - Bacteriophage MS2 displays unreported capsid variability assembling T = 4 and mixed capsids
T2  - Molecular Microbiology
UR  - http://dx.doi.org/10.1111/mmi.14406
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31618483
UR  - https://onlinelibrary.wiley.com/doi/full/10.1111/mmi.14406
UR  - http://hdl.handle.net/10044/1/74539
VL  - 113
ER  -
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