Imperial College London
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ProfessorPaulFreemont

Faculty of Medicine, Department of Infectious Disease

Chair in Protein Crystallography
 
 
 
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Contact

 

+44 (0)20 7594 5327p.freemont

 
 
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Location

 

259Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Florea:2016:10.1038/srep23635,
author = {Florea, M and Reeve, B and Abbott, J and Freemont, PS and Ellis, T},
doi = {10.1038/srep23635},
journal = {Scientific Reports},
title = {Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582.},
url = {http://dx.doi.org/10.1038/srep23635},
volume = {6},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Bacterial cellulose is a strong, highly pure form of cellulose that is used in a range of applications in industry, consumer goods and medicine. Gluconacetobacter hansenii ATCC 53582 is one of the highest reported bacterial cellulose producing strains and has been used as a model organism in numerous studies of bacterial cellulose production and studies aiming to increased cellulose productivity. Here we present a high-quality draft genome sequence for G. hansenii ATCC 53582 and find that in addition to the previously described cellulose synthase operon, ATCC 53582 contains two additional cellulose synthase operons and several previously undescribed genes associated with cellulose production. In parallel, we also develop optimized protocols and identify plasmid backbones suitable for transformation of ATCC 53582, albeit with low efficiencies. Together, these results provide important information for further studies into cellulose synthesis and for future studies aiming to genetically engineer G. hansenii ATCC 53582 for increased cellulose productivity.
AU  - Florea,M
AU  - Reeve,B
AU  - Abbott,J
AU  - Freemont,PS
AU  - Ellis,T
DO  - 10.1038/srep23635
PY  - 2016///
SN  - 2045-2322
TI  - Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582.
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/srep23635
UR  - http://hdl.handle.net/10044/1/30671
VL  - 6
ER  -
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