Imperial College London
Alternate

Dr. Patrik R. Jones

Faculty of Natural Sciences, Department of Life Sciences

Professor of Metabolic Engineering
 
 
 
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Contact

 

+44 (0)20 7594 5213p.jones

 
 
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Location

 

503Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Malatinszky:2017:10.1104/pp.16.01487,
author = {Malatinszky, D and Steuer, R and Jones, PR},
doi = {10.1104/pp.16.01487},
journal = {Plant Physiology},
pages = {509--523},
title = {A comprehensively curated genome-scale two-cell model for the cyanobacterium Anabaena sp. PCC 7120},
url = {http://dx.doi.org/10.1104/pp.16.01487},
volume = {173},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Anabaena sp.  PCC  7120  is  a  nitrogen-fixing  filamentous  cyanobacterium. Under  nitrogen limiting conditions, a fraction of the vegetative cells in each filament terminally differentiate to  non-growing  heterocysts.  Heterocysts  are  metabolically  and  structurally  specialized  to enable O2 -sensitive nitrogen fixation. The functionality of the filament, as an association of vegetative cells and heterocysts, is postulated to depend on metabolic exchange of electrons, carbon and fixed nitrogen. In the present work, we compile and evaluate a comprehensive curated stoichiometric model of this two-cell system, with the objective function based on the growth  of  the  filament  under  diazotrophic  conditions.  The  predicted  growth  rate  under nitrogen replete and deplete conditions, as well as the effect of external carbon and nitrogen sources,  was  thereafter  verified.  Furthermore,  the  model  was  utilized  to  comprehensively evaluate  the  optimality  of  putative  metabolic  exchange  reactions  between  heterocysts  and vegetative  cells. The  model suggested that optimal growth requires  at least four  exchange metabolites. Several combinations of exchange metabolites resulted in predicted growth rates that  are  higher  than  growth  rates  achieved  by  only  considering exchange  of  metabolites previously  suggested  in  the  literature.  The  curated  model  of  the  metabolic  network  of Anabaena  sp. PCC  7120 enhances  our ability to understand the  metabolic organization of multi-cellular cyanobacteria and provides a platform for further study and engineering of their metabolism
AU  - Malatinszky,D
AU  - Steuer,R
AU  - Jones,PR
DO  - 10.1104/pp.16.01487
EP  - 523
PY  - 2017///
SN  - 0032-0889
SP  - 509
TI  - A comprehensively curated genome-scale two-cell model for the cyanobacterium Anabaena sp. PCC 7120
T2  - Plant Physiology
UR  - http://dx.doi.org/10.1104/pp.16.01487
UR  - http://hdl.handle.net/10044/1/42795
VL  - 173
ER  -
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