Imperial College London
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ProfessorPeterKohl

Faculty of Medicine, National Heart & Lung Institute

Visiting Professor
 
 
 
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Contact

 

p.kohl Website

 
 
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Location

 

Heart Science CentreHarefield Hospital

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Summary

 

Publications

Citation

BibTex format

@article{Johnston:2017:10.1016/j.pbiomolbio.2017.09.014,
author = {Johnston, CM and Rog-Zielinska, EA and Wulfers, EM and Houwaart, T and Siedlecka, U and Knopfel, T and Kohl, P and Schneider-Warme, F},
doi = {10.1016/j.pbiomolbio.2017.09.014},
journal = {Progress in Biophysics and Molecular Biology},
pages = {140--149},
title = {Optogenetic targeting of cardiac myocytes and non-myocytes: tools, challenges and utility},
url = {http://dx.doi.org/10.1016/j.pbiomolbio.2017.09.014},
volume = {130},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In optogenetics, light-activated proteins are used to monitor and modulate cellular behaviour with light. Combining genetic targeting of distinct cellular populations with defined patterns of optical stimulation enables one to study specific cell classes in complex biological tissues. In the current study we attempted to investigate the functional relevance of heterocellular electrotonic coupling in cardiac tissue in situ. In order to do that, we used a Cre-Lox approach to express the light-gated cation channel Channelrhodopsin-2 (ChR2) specifically in either cardiac myocytes or non-myocytes. Despite high specificity when using the same Cre driver lines in a previous study in combination with a different optogenetic probe, we found patchy off-target ChR2 expression in cryo-sections and extended z-stack imaging through the ventricular wall of hearts cleared using CLARITY. Based on immunohistochemical analysis, single-cell electrophysiological recordings and whole-genome sequencing, we reason that non-specificity is caused on the Cre recombination level. Our study highlights the importance of careful design and validation of the Cre recombination targets for reliable cell class specific expression of optogenetic tools.
AU  - Johnston,CM
AU  - Rog-Zielinska,EA
AU  - Wulfers,EM
AU  - Houwaart,T
AU  - Siedlecka,U
AU  - Knopfel,T
AU  - Kohl,P
AU  - Schneider-Warme,F
DO  - 10.1016/j.pbiomolbio.2017.09.014
EP  - 149
PY  - 2017///
SN  - 0079-6107
SP  - 140
TI  - Optogenetic targeting of cardiac myocytes and non-myocytes: tools, challenges and utility
T2  - Progress in Biophysics and Molecular Biology
UR  - http://dx.doi.org/10.1016/j.pbiomolbio.2017.09.014
UR  - http://hdl.handle.net/10044/1/54812
VL  - 130
ER  -
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