The research in my group focuses on respiratory and/or meningitis-causing pathogens of man, e.g. Neisseria meningitidis, Haemophilus influenzae and Mycobacterium tuberculosis, and animals e.g. Actinobacillus pleuropneumoniae (APP), Haemophilus parasuis, Pasteurella multocida. Dependent on the pathogen, the aims are to improve vaccines, diagnostics, therapeutics and a greater understanding of the basis of bacterial pathogenicity. A major focus of our work is on the pig pathogen APP carried out in collaboration with Professor Andrew Rycroft (Royal Veterinary College London). We have developed high throughput genome wide mutagenesis protocols and screens (based on Transposon Directed Insertion site Sequencing [TraDIS]) to identify APP genes essential for growth in vitro and in vivo, with a view to developing novel antimicrobial and vaccine strategies. The methods developed are applicable to other pathogenic members of the Pasteurellaceae i.e. Aggregatibacter actinomycetemcomitans, H. influenzae, Mannheimia haemolytica, and P. multocida.
In collaboration with Dr Anastasia Callaghan (University of Portsmouth) and Professor Denise Bazzolli (University of Viçosa, Brazil) we are evaluating whether inhibition of sRNAs of APP is a viable control strategy. Other collaborations include that with the UK Animal and Plant Health Agency (APHA) and the Wellcome Trust Sanger Centre, where we have identified in APP (1) plasmid borne dfr14 genes (mediating resistance to trimethoprim), the first description in any member of the Pasteurellaceae; and (2) sequenced plasmids mediating florfenicol resistance and ampicillin. Additionally, in a collaboration involving Drs Ee (National University of Singapore) and Yang (Institute of Bioengineering and Nanotechnology, Singapore), and Drs Sandra Newton and Brian Robertson (Imperial College London), rationally designed antimicrobial peptides are being tested for their efficacy in vitro and in vivo to inhibit M. tuberculosis.
In addition, we are seeking vaccine candidates that will prevent disease caused by Neisseria meningitidis serogroup B infections. Monoclonal antibodies are derived from patients recovering from meningococcal disease, and the proteins recognised on the surface of the bacterium determined.
et al., 2020, Basal Level Effects of (p)ppGpp in the Absence of Branched Chain Amino Acids in Actinobacillus pleuropneumoniae., J Bacteriol
et al., Draft genome sequences of the type strains of Actinobacillus indolicus (46K2C) and Actinobacillus porcinus (NM319), two NAD-dependent bacterial species found in the respiratory tract of pigs, Microbiology Resource Announcements, ISSN:2576-098X
et al., 2019, Free serum haemoglobin is associated with brain atrophy in secondary progressive multiple sclerosis [version 2; peer review: 3 approved], Wellcome Open Research, Vol:1, ISSN:2398-502X
et al., 2019, Publisher Correction: Genomic signatures of human and animal disease in the zoonotic pathogen Streptococcus suis (vol 6, 6740, 2015), Nature Communications, Vol:10, ISSN:2041-1723
et al., 2019, Galleria mellonella: a novel infection model for screening potential anti-mycobacterial compounds against members of the Mycobacterium tuberculosis complex, Frontiers in Microbiology, Vol:10, ISSN:1664-302X