Imperial College London
Alternate

ProfessorPaulLangford

Faculty of Medicine, Department of Infectious Disease

Professor of Paediatric Infectious Diseases
 
 
 
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Contact

 

+44 (0)20 7594 3359p.langford Website

 
 
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Location

 

236Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Asai:2019:10.3791/59703,
author = {Asai, M and Li, Y and Khara, J and Gladstone, C and Robertson, B and Langford, P and Newton, S},
doi = {10.3791/59703},
journal = {Jove-Journal of Visualized Experiments},
title = {Use of the invertebrate Galleria Mellonella as an infection model to study the Mycobacterium tuberculosis complex},
url = {http://dx.doi.org/10.3791/59703},
volume = {148},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Tuberculosis is the leading global cause of infectious disease mortality and roughly a quarter of the world’s population is believed to be infected with Mycobacterium tuberculosis. Despite decades of research, many of the mechanisms behind the success of M. tuberculosis as a pathogenic organism remain to be investigated, and the development of safer, more effective antimycobacterial drugs are urgently needed to tackle the rise and spread of drug resistant tuberculosis. However, the progression of tuberculosis research is bottlenecked by traditional  mammalian infection models that are expensive, time consuming, and ethically challenging.Previously we established the larvae of the insect Galleria mellonella (greater wax moth) as a novel, reproducible, low cost, high-throughput and ethically acceptable infection model for members of the M. tuberculosis complex. Here we describe the maintenance, preparation, and infection of G. mellonella with bioluminescent Mycobacterium bovis BCG lux. Using this infection model, mycobacterial dose dependent virulence can be observed, and a rapid readout of in vivo mycobacterial burden using bioluminescence measurements is easily achievable and reproducible. Although limitations exist, such as the lack of a fully annotated genome for transcriptomic analysis, ontological analysis against genetically similar insects can be carried out. As a low cost, rapid, and ethically acceptable model for tuberculosis, G. mellonella can be used as a pre-screen to determine drug efficacy and toxicity, and to determine comparative mycobacterial virulence prior to the use of conventional mammalian models. The use of the G. mellonella-mycobacteria model will lead to a reduction in the substantial number of animals currently used in tuberculosis research.
AU  - Asai,M
AU  - Li,Y
AU  - Khara,J
AU  - Gladstone,C
AU  - Robertson,B
AU  - Langford,P
AU  - Newton,S
DO  - 10.3791/59703
PY  - 2019///
SN  - 1940-087X
TI  - Use of the invertebrate Galleria Mellonella as an infection model to study the Mycobacterium tuberculosis complex
T2  - Jove-Journal of Visualized Experiments
UR  - http://dx.doi.org/10.3791/59703
UR  - http://hdl.handle.net/10044/1/67656
VL  - 148
ER  -
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