Imperial College London
Alternate

ProfessorPaulLangford

Faculty of Medicine, Department of Infectious Disease

Professor of Paediatric Infectious Diseases
 
 
 
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Contact

 

+44 (0)20 7594 3359p.langford Website

 
 
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Location

 

236Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Li:2020:10.1128/JB.00640-19,
author = {Li, G and Zhao, Q and Luan, T and Hu, Y and Zhang, Y and Li, T and Wang, C and Xie, F and Zhang, W and Langford, PR and Liu, S},
doi = {10.1128/JB.00640-19},
journal = {Journal of Bacteriology},
pages = {1--14},
title = {Basal level effects of (p)ppGpp in the absence of branched chain amino acids in acctinobacillus pleuropneumoniae},
url = {http://dx.doi.org/10.1128/JB.00640-19},
volume = {202},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The (p)ppGpp-mediated stringent response (SR) is a highly conserved regulatory mechanism in bacterial pathogens, enabling adaptation to adverse environments and linked to pathogenesis. Actinobacillus pleuropneumoniae can cause damage to the lungs of pigs, it's only known natural host. Pig lungs are known to have a low concentration of free branched chain amino acids (BCAAs) compared to plasma. We had investigated the role for (p)ppGpp in viability and biofilm formation of A. pleuropneumoniae Now, we sought to determine whether (p)ppGpp was a trigger signal for the SR in A. pleuropneumoniae in the absence of BCAAs. Combining transcriptome and phenotypic analyses of wild type (WT) and relAspoT double mutant (which does not produce (p)ppGpp), we found that (p)ppGpp could repress de novo purine biosynthesis and activate antioxidant pathways. There was a positive correlation between GTP and endogenous hydrogen peroxide content. Furthermore, the growth, viability, morphology and virulence were altered by the inability to produce (p)ppGpp. Genes involved in the biosynthesis of BCAAs were constitutively up-regulated regardless of the existence of BCAAs without accumulation of (p)ppGpp beyond basal level. Collectively, our study shows that the absence of BCAAs was not a sufficient signal to trigger the SR in A. pleuropneumoniae (p)ppGpp-mediated regulation in A. pleuropneumoniae is different to that described for the model organism Escherichia coli. Further work will establish whether the (p)ppGpp-dependent SR mechanism in Apleuropneumoniae is conserved among other veterinary pathogens, especially those in the Pasteurellaceae family.IMPORTANCE(p)ppGpp is a key player in reprogramming transcriptomes to respond to nutritional challenges. Here, we present a transcriptional and phenotypic differences of A. pleuropneumoniae grown in different chemically defined media in the absence of (p)ppGpp. We show that the deprivation of branch-chain amino acids (BCAAs) does not elicit a cha
AU  - Li,G
AU  - Zhao,Q
AU  - Luan,T
AU  - Hu,Y
AU  - Zhang,Y
AU  - Li,T
AU  - Wang,C
AU  - Xie,F
AU  - Zhang,W
AU  - Langford,PR
AU  - Liu,S
DO  - 10.1128/JB.00640-19
EP  - 14
PY  - 2020///
SN  - 0021-9193
SP  - 1
TI  - Basal level effects of (p)ppGpp in the absence of branched chain amino acids in acctinobacillus pleuropneumoniae
T2  - Journal of Bacteriology
UR  - http://dx.doi.org/10.1128/JB.00640-19
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32015147
UR  - https://journals.asm.org/doi/10.1128/JB.00640-19
UR  - http://hdl.handle.net/10044/1/102159
VL  - 202
ER  -
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