Imperial College London
Alternate

ProfessorPaulLangford

Faculty of Medicine, Department of Infectious Disease

Professor of Paediatric Infectious Diseases
 
 
 
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Contact

 

+44 (0)20 7594 3359p.langford Website

 
 
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Location

 

236Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Eberle:2020:10.1128/IAI.00879-19,
author = {Eberle, KC and Hau, SJ and Luan, S-L and Weinert, LA and Stasko, JA and Wang, J and Peters, SE and Langford, PR and Rycroft, AN and Wren, BW and Maskell, DJ and Tucker, AW and Brockmeier, SL},
doi = {10.1128/IAI.00879-19},
journal = {Infection and Immunity},
pages = {1--11},
title = {Generation and evaluation of a glaesserella (Haemophilus) parasuis capsular mutant},
url = {http://dx.doi.org/10.1128/IAI.00879-19},
volume = {88},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Glaesserella (Haemophilus) parasuis is a commensal bacterium of the upper respiratory tract in pigs and also the causative agent of Glässer’s disease, which causes significant morbidity and mortality in pigs worldwide. Isolates are characterized into 15 serovars by their capsular polysaccharide, which has shown a correlation with isolate pathogenicity. To investigate the role the capsule plays in G. parasuis virulence and host interaction, a capsule mutant of the serovar 5 strain HS069 was generated (HS069Δcap) through allelic exchange following natural transformation. HS069Δcap was unable to cause signs of systemic disease during a pig challenge study and had increased sensitivity to complement killing and phagocytosis by alveolar macrophages. Compared with the parent strain, HS069Δcap produced more robust biofilm and adhered equivalently to 3D4/31 cells; however, it was unable to persistently colonize the nasal cavity of inoculated pigs, with all pigs clearing HS069Δcap by 5 days postchallenge. Our results indicate the importance of the capsular polysaccharide to G. parasuis virulence as well as nasal colonization in pigs.
AU  - Eberle,KC
AU  - Hau,SJ
AU  - Luan,S-L
AU  - Weinert,LA
AU  - Stasko,JA
AU  - Wang,J
AU  - Peters,SE
AU  - Langford,PR
AU  - Rycroft,AN
AU  - Wren,BW
AU  - Maskell,DJ
AU  - Tucker,AW
AU  - Brockmeier,SL
DO  - 10.1128/IAI.00879-19
EP  - 11
PY  - 2020///
SN  - 0019-9567
SP  - 1
TI  - Generation and evaluation of a glaesserella (Haemophilus) parasuis capsular mutant
T2  - Infection and Immunity
UR  - http://dx.doi.org/10.1128/IAI.00879-19
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000528224200003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://journals.asm.org/doi/10.1128/IAI.00879-19
UR  - http://hdl.handle.net/10044/1/103201
VL  - 88
ER  -
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