Imperial College London
Alternate

ProfessorPaulLangford

Faculty of Medicine, Department of Infectious Disease

Professor of Paediatric Infectious Diseases
 
 
 
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Contact

 

+44 (0)20 7594 3359p.langford Website

 
 
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Location

 

236Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Siris:2021:10.1007/978-1-0716-0795-4_2,
author = {Siris, S and Gladstone, CA and Guo, Y and Pinder, CL and Shattock, RJ and McKay, PF and Langford, PR and Bidmos, FA},
doi = {10.1007/978-1-0716-0795-4_2},
journal = {Methods Mol Biol},
pages = {9--18},
title = {Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) Using Bacterial Whole Cells as Molecular Probes.},
url = {http://dx.doi.org/10.1007/978-1-0716-0795-4_2},
volume = {2183},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The immunoglobulin capture assay (ICA) enables the enrichment for pathogen-specific plasmablasts from individuals with a confirmed adaptive immune response to vaccination or disseminated infection. Only single recombinant antigens have been used previously as probes in this ICA and it was unclear whether the method was applicable to complex probes such as whole bacterial cells. Here, we describe the enrichment of plasmablasts specific for polysaccharide and protein antigens of both Streptococcus pneumoniae and Neisseria meningitidis using whole formalin-fixed bacterial cells as probes. The modified ICA protocol described here allowed for a pathogen-specific hmAb cloning efficiency of >80%.
AU  - Siris,S
AU  - Gladstone,CA
AU  - Guo,Y
AU  - Pinder,CL
AU  - Shattock,RJ
AU  - McKay,PF
AU  - Langford,PR
AU  - Bidmos,FA
DO  - 10.1007/978-1-0716-0795-4_2
EP  - 18
PY  - 2021///
SP  - 9
TI  - Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) Using Bacterial Whole Cells as Molecular Probes.
T2  - Methods Mol Biol
UR  - http://dx.doi.org/10.1007/978-1-0716-0795-4_2
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32959237
VL  - 2183
ER  -
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