Imperial College London
Alternate

ProfessorPaulLangford

Faculty of Medicine, Department of Infectious Disease

Professor of Paediatric Infectious Diseases
 
 
 
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Contact

 

+44 (0)20 7594 3359p.langford Website

 
 
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Location

 

236Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Menikou:2020:10.1371/journal.pone.0244157,
author = {Menikou, S and McArdle, AJ and Li, M-S and Kaforou, M and Langford, PR and Levin, M},
doi = {10.1371/journal.pone.0244157},
journal = {PLoS One},
title = {A proteomics-based method for identifying antigens within immune complexes},
url = {http://dx.doi.org/10.1371/journal.pone.0244157},
volume = {15},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A novel approach to recover and identify immune complexes (ICs) was developed using size exclusion chromatography (SEC) and affinity chromatography on immunoglobulin binding columns (HiTrap Protein G). The purification process was monitored by 1D SDS-PAGE, protein staining, Western blotting and, finally, liquid chromatography tandem mass spectrometry (LC MS/MS) was used to identify the recovered antigens. This approach was applied to serum with artificially created immune complexes (ICs) comprising vaccine antigen (influenza) and antibody, which led to recovery and identification of influenza peptides within the recovered ICs. This approach was compared with the established method for IC detection and recovery, polyethylene glycol (PEG) precipitation, followed by LC MS/MS. Both approaches successfully enabled capture, recovery and characterization of immunoglobulins and influenza antigen(s) in complex with the immunoglobulins. However, PEG precipitation has the advantage of simplicity and is more suited for large scale studies.
AU  - Menikou,S
AU  - McArdle,AJ
AU  - Li,M-S
AU  - Kaforou,M
AU  - Langford,PR
AU  - Levin,M
DO  - 10.1371/journal.pone.0244157
PY  - 2020///
SN  - 1932-6203
TI  - A proteomics-based method for identifying antigens within immune complexes
T2  - PLoS One
UR  - http://dx.doi.org/10.1371/journal.pone.0244157
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000603071600017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/86117
VL  - 15
ER  -
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