Imperial College London
Alternate

Dr Paul F. McKay

Faculty of Medicine, Department of Infectious Disease

Advanced Research Fellow
 
 
 
//

Contact

 

+44 (0)20 7594 2542p.mckay

 
 
//

Location

 

125 (Shattock Group)Wright Fleming WingSt Mary's Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Blakney:2018:10.1021/acs.biomac.8b00429,
author = {Blakney, AK and Yilmaz, G and McKay, PF and Becer, CR and Shattock, RJ},
doi = {10.1021/acs.biomac.8b00429},
journal = {Biomacromolecules},
pages = {2870--2879},
title = {One size does not fit all: The effect of chain length and charge density of poly(ethylene imine) based copolymers on delivery of pDNA, mRNA, and repRNA polyplexes.},
url = {http://dx.doi.org/10.1021/acs.biomac.8b00429},
volume = {19},
year = {2018}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Nucleic acid delivery systems are commonly translated between different modalities, such as DNA and RNA of varying length and structure, despite physical differences in these molecules that yield disparate delivery efficiency with the same system. Here, we synthesized a library of poly(2-ethyl-2-oxazoline)/poly(ethylene imine) copolymers with varying molar mass and charge densities in order to probe how pDNA, mRNA, and RepRNA polyplex characteristics affect transfection efficiency. The library was utilized in a full factorial design of experiment (DoE) screening, with outputs of luciferase expression, particle size, surface charge, and particle concentration. The optimal copolymer molar mass and charge density was found as 83 kDa/100%, 72 kDa/100%, and 45 kDa/80% for pDNA, RepRNA, and mRNA, respectively. While 10 of the synthesized copolymers enhanced the transfection efficiency of pDNA and mRNA, only 2 copolymers enhanced RepRNA transfection efficiency, indicating a narrow and more stringent design space for RepRNA. These findings suggest that there is not a "one size fits all" polymer for different nucleic acid species.
AU  - Blakney,AK
AU  - Yilmaz,G
AU  - McKay,PF
AU  - Becer,CR
AU  - Shattock,RJ
DO  - 10.1021/acs.biomac.8b00429
EP  - 2879
PY  - 2018///
SN  - 1525-7797
SP  - 2870
TI  - One size does not fit all: The effect of chain length and charge density of poly(ethylene imine) based copolymers on delivery of pDNA, mRNA, and repRNA polyplexes.
T2  - Biomacromolecules
UR  - http://dx.doi.org/10.1021/acs.biomac.8b00429
UR  - https://www.ncbi.nlm.nih.gov/pubmed/29698602
UR  - https://pubs.acs.org/doi/10.1021/acs.biomac.8b00429
UR  - http://hdl.handle.net/10044/1/59807
VL  - 19
ER  -
Download