Publications
242 results found
de Paula Alves Sousa A, Johnson KR, Nicholas R, et al., 2016, Intrathecal T-cell clonal expansions in patients with multiple sclerosis, Annals of Clinical and Translational Neurology, Vol: 3, Pages: 422-433, ISSN: 2328-9503
ObjectiveAnalysis of the T-cell receptor (TCR) repertoire in the cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) can reveal antigen-specific immune responses potentially implicated in the disease process. We applied a new unbiased deep-sequencing method for TCR repertoire analysis to accurately measure and compare receptor diversity and clonal expansions within the peripheral and CSF-trafficking T-cell populations of patients with MS and control individuals with idiopathic intracranial hypertension (IIH).MethodsPaired blood and CSF TCR β-chain libraries from five MS patients and five IIH controls were sequenced, yielding a total of 80 million reads.ResultsAlthough TCR repertoire diversity was greater in the blood and CSF compartments of MS patients when compared with IIH controls, it is notable that the frequency of clonal expansions was also significantly higher in both compartments of MS patients. Highly expanded T-cell clones were enriched in the CSF compartment of MS patients compared to peripheral blood, very few of them were detected in both compartments.InterpretationCollectively, our data provide a proof of principle that private compartmentalized T-cell expansion exists in the intrathecal space of MS patients.
Sormani MP, Muraro P, 2016, Updated views on autologous hematopoietic stem cell transplantation for treatment of multiple sclerosis, Expert Review of Neurotherapeutics, Vol: 16, Pages: 469-470, ISSN: 1744-8360
Nash R, Hutton G, Racke M, et al., 2016, Five-year outcomes of halt-MS: high-dose immunosuppressive therapy and autologous hematopoietic cell transplantation for severe relapsing-remitting multiple sclerosis, 42nd Annual Meeting of the European Society for Blood and Marrow Transplantation, Publisher: Nature Publishing Group, Pages: S61-S62, ISSN: 1476-5365
Collins F, Nicholas R, Malik O, et al., 2016, The role of Autologous Haematopoietic Stem Cell Transplant in MS - Updated results from the Pan-London MS group, 42nd Annual Meeting of the European-Society-for-Blood-and-Marrow-Transplantation, Publisher: NATURE PUBLISHING GROUP, Pages: S494-S495, ISSN: 0268-3369
Scalfari A, Lederer C, Daumer M, et al., 2016, The relationship of age with the clinical phenotype in multiple sclerosis, MULTIPLE SCLEROSIS JOURNAL, Vol: 22, Pages: 1750-1758, ISSN: 1352-4585
Colasanti A, Guo, Giannetti P, et al., 2015, Hippocampal neuroinflammation, functional connectivity and depressive symptoms in multiple sclerosis, Biological Psychiatry, Vol: 80, Pages: 62-72, ISSN: 1873-2402
BackgroundDepression, a condition commonly comorbid with multiple sclerosis (MS), is associated more generally with elevated inflammatory markers and hippocampal pathology. We hypothesized that neuroinflammation in the hippocampus is responsible for depression associated with MS. We characterized the relationship between depressive symptoms and hippocampal microglial activation in patients with MS using the 18-kDa translocator protein radioligand [18F]PBR111. To evaluate pathophysiologic mechanisms, we explored the relationships between hippocampal neuroinflammation, depressive symptoms, and hippocampal functional connectivities defined by resting-state functional magnetic resonance imaging.MethodsThe Beck Depression Inventory (BDI) was administered to 11 patients with MS and 22 healthy control subjects before scanning with positron emission tomography and functional magnetic resonance imaging. We tested for higher [18F]PBR111 uptake in the hippocampus of patients with MS relative to healthy control subjects and examined the correlations between [18F]PBR111 uptake, BDI scores, and hippocampal functional connectivities in the patients with MS.ResultsPatients with MS had an increased hippocampal [18F]PBR111 distribution volume ratio relative to healthy control subjects (p = .024), and the hippocampal distribution volume ratio was strongly correlated with the BDI score in patients with MS (r = .86, p = .006). Hippocampal functional connectivities to the subgenual cingulate and prefrontal and parietal regions correlated with BDI scores and [18F]PBR111 distribution volume ratio.ConclusionsOur results provide evidence that hippocampal microglial activation in MS impairs the brain functional connectivities in regions contributing to maintenance of a normal affective state. Our results suggest a rationale for the responsiveness of depression in some patients with MS to effective control of brain neuroinflammation. Our findings also lend support to further investigation of t
Colasanti A, Guo Q, Giannetti P, et al., 2015, Hippocampal inflammation and depression in multiple sclerosis: integrating evidence from TSPO PET and resting state fMRI, 31st Congress of the European-Committee-for-Treatment-and-Research-in-Multiple-Sclerosis (ECTRIMS), Publisher: SAGE Publications (UK and US), Pages: 492-493, ISSN: 1477-0970
Sousa ADPA, Jonhson KR, Nicholas R, et al., 2015, Expanded T-cell clonotype sequences showed specificity relatedness in the peripheral blood of MS patients by phylogenetic tree analysis, 31st Congress of the European-Committee-for-Treatment-and-Research-in-Multiple-Sclerosis (ECTRIMS), Publisher: SAGE PUBLICATIONS LTD, Pages: 442-442, ISSN: 1352-4585
Ali R, Nicholas R, Marley S, et al., 2015, Preliminary results of a phase 2 trial of autologous mesenchymal cell therapy in MS (STREAMS), 31st Congress of the European-Committee-for-Treatment-and-Research-in-Multiple-Sclerosis (ECTRIMS), Publisher: SAGE PUBLICATIONS LTD, Pages: 556-556, ISSN: 1352-4585
Scalfari A, Christian L, Daumer M, et al., 2015, The effect of age on the disease phenotype before the onset of progressive MS, 31st Congress of the European-Committee-for-Treatment-and-Research-in-Multiple-Sclerosis (ECTRIMS), Publisher: SAGE PUBLICATIONS LTD, Pages: 93-93, ISSN: 1352-4585
Muraro PA, Pasquini M, Atkins H, et al., 2015, Long term outcomes after autologous hematopoietic stem cell transplantation for treatment of MS, 31st Congress of the European-Committee-for-Treatment-and-Research-in-Multiple-Sclerosis (ECTRIMS), Publisher: SAGE PUBLICATIONS LTD, Pages: 63-64, ISSN: 1352-4585
Scalfari A, Mattoscio M, Sormani MP, et al., 2015, The cortical damage among MS patients with frequent early relapses, 31st Congress of the European-Committee-for-Treatment-and-Research-in-Multiple-Sclerosis (ECTRIMS), Publisher: SAGE PUBLICATIONS LTD, Pages: 475-476, ISSN: 1352-4585
Uccelli A, Brundin L, Clanet M, et al., 2015, MESEMS: a randomized, double blind placebo-controlled cross-over study to evaluate safety and efficacy of intravenous administration of autologous mesenchymal stem cells in patients with multiple sclerosis, 31st Congress of the European-Committee-for-Treatment-and-Research-in-Multiple-Sclerosis (ECTRIMS), Publisher: SAGE PUBLICATIONS LTD, Pages: 559-560, ISSN: 1352-4585
Sousa ADPA, Johnson KR, Ohayon J, et al., 2015, Deep sequencing of the TCRβ repertoire reveals T cell clonal expansion is associated with HTLV-1 proviral load in HAM/TSP patients, RETROVIROLOGY, Vol: 12, ISSN: 1742-4690
Scalfari A, Lederer C, Daumer M, et al., 2015, The influence of age on the disease phenotype preceding the onset of progressive multiple sclerosis, 1st Congress of the European-Academy-of-Neurology, Publisher: WILEY, Pages: 301-301, ISSN: 1351-5101
Scalfari A, Ebers G, Nicholas R, et al., 2015, Frequent early relapses and severe cortical damage characterize patients at high risk of early developing progressive multiple sclerosis, 1st Congress of the European-Academy-of-Neurology, Publisher: WILEY, Pages: 620-620, ISSN: 1351-5101
Mattoscio M, Nicholas R, Sormani MP, et al., 2015, Hematopoietic mobilization Potential biomarker of response to natalizumab in multiple sclerosis, NEUROLOGY, Vol: 84, Pages: 1473-1482, ISSN: 0028-3878
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- Citations: 23
Muraro PA, 2015, <i>Andiamo</i>! Moving forward with autologous hematopoietic transplantation for highly active MS, NEUROLOGY, Vol: 84, Pages: 968-969, ISSN: 0028-3878
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- Citations: 2
Clay J, Kelsey P, Bell S, et al., 2015, Autologous haematopoietic stem cell transplantation in aggressive multiple sclerosis: a UK cohort from two centres, 41st Annual Meeting of the European-Society-for-Blood-and-Marrow-Transplantation, Publisher: NATURE PUBLISHING GROUP, Pages: S61-S61, ISSN: 0268-3369
Nash RA, Hutton GJ, Racke MK, et al., 2015, High-Dose Immunosuppressive Therapy and Autologous Hematopoietic Cell Transplantation for Relapsing-Remitting Multiple Sclerosis (HALT-MS) A 3-Year Interim Report, JAMA NEUROLOGY, Vol: 72, Pages: 159-169, ISSN: 2168-6149
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- Citations: 122
Alexander T, Bondanza A, Muraro PA, et al., 2015, SCT for severe autoimmune diseases: consensus guidelines of the European Society for Blood and Marrow Transplantation for immune monitoring and biobanking, BONE MARROW TRANSPLANTATION, Vol: 50, Pages: 173-180, ISSN: 0268-3369
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- Citations: 61
Sousa ADPA, Malmegrim KCR, Panepucci RA, et al., 2015, Autologous haematopoietic stem cell transplantation reduces abnormalities in the expression of immune genes in multiple sclerosis, CLINICAL SCIENCE, Vol: 128, Pages: 111-U57, ISSN: 0143-5221
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- Citations: 26
Sousa ADPA, Nicholas R, Johnson K, et al., 2014, Deep sequencing of T-cell receptor repertoire reveals enrichment of highly expanded clonotypes in cerebrospinal fluid from multiple sclerosis, 12th International Congress of Neuroimmunology (ISNI), Publisher: ELSEVIER SCIENCE BV, Pages: 197-198, ISSN: 0165-5728
Ali R, Nicolas R, Marley S, et al., 2014, STREAMS: PRELIMINARY RESULTS FROM A PHASE II TRIAL, Meeting of the Associatiion-of-British-Neurologists, Publisher: BMJ PUBLISHING GROUP, ISSN: 0022-3050
Saso A, Muraro P, Nicholas R, 2014, ASSOCIATING AUTOANTIBODIES WITH CLINICAL FEATURES IN MS, Meeting of the Associatiion-of-British-Neurologists, Publisher: BMJ PUBLISHING GROUP, ISSN: 0022-3050
Nicholas R, Silber E, Brex P, et al., 2014, AUTOLOGOUS HAEMATOPOIETIC STEM CELL TRANSPLANTATION IN MS, Meeting of the Associatiion-of-British-Neurologists, Publisher: BMJ PUBLISHING GROUP, ISSN: 0022-3050
Khoder A, Sarvaria A, Alsuliman A, et al., 2014, Regulatory B cells are enriched within the IgM memory and transitional subsets in healthy donors but are deficient in chronic GVHD, Blood, Vol: 124, Pages: 2034-2045, ISSN: 0006-4971
A subset of regulatory B cells (Bregs) in mice negatively regulate T-cell immune responses through the secretion of regulatory cytokines such as IL-10 and direct cell-cell contact and have been linked to experimental models of autoimmunity, inflammation, and cancer. However, the regulatory function of Bregs in human disease is much less clear. Here we demonstrate that B cells with immunoregulatory properties are enriched within both the CD19+IgM+CD27+ memory and CD19+CD24hiCD38hi transitional B-cell subsets in healthy human donors. Both subsets suppressed the proliferation and interferon-γ production of CD3/CD28-stimulated autologous CD4+ T cells in a dose-dependent manner, and both relied on IL-10 secretion as well as cell-cell contact, likely mediated through CD80 and CD86, to support their full suppressive function. Moreover, after allogeneic stem cell transplantation, Bregs from patients with chronic graft-versus-host disease (cGVHD) were less frequent and less likely to produce IL-10 than were Bregs from healthy donors and patients without cGVHD. These findings suggest that Bregs may be involved in the pathogenesis of cGVHD and support future investigation of regulatory B cell–based therapy in the treatment of this disease.
Sousa ADPA, Nicholas R, Johnson K, et al., 2014, Deep sequencing of T-cell receptor repertoire reveals enrichment of highly expanded clonotypes in cerebrospinal fluid from multiple sclerosis patients, Joint ACTRIMS-ECTRIMS Meeting, Publisher: SAGE PUBLICATIONS LTD, Pages: 57-57, ISSN: 1352-4585
Colasanti A, Guo Q, Muhlert N, et al., 2014, In Vivo Assessment of Brain White Matter Inflammation in Multiple Sclerosis with <SUP>18</SUP>F-PBR111 PET, JOURNAL OF NUCLEAR MEDICINE, Vol: 55, Pages: 1112-1118, ISSN: 0161-5505
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- Citations: 78
Robins H, Muraro P, Malhotra S, et al., 2014, T cell repertoire renewal following autologous stem cell transplantation for multiple sclerosis, JOURNAL OF IMMUNOLOGY, Vol: 192, ISSN: 0022-1767
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