Publications
173 results found
Boehm M, Yu J, Reisinger V, et al., 2012, Subunit composition of CP43-less photosystem II complexes of <i>Synechocystis</i> sp PCC 6803: implications for the assembly and repair of photosystem II, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 367, Pages: 3444-3454, ISSN: 0962-8436
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- Citations: 52
Burgess SJ, Tredwell G, Molnar A, et al., 2012, Artificial microRNA-mediated knockdown of pyruvate formate lyase (PFL1) provides evidence for an active 3-hydroxybutyrate production pathway in the green alga <i>Chlamydomonas reinhardtii</i>, JOURNAL OF BIOTECHNOLOGY, Vol: 162, Pages: 57-66, ISSN: 0168-1656
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- Citations: 18
Mullineaux CW, Liu LN, Bryan SJ, et al., 2012, Sub-micron scale distribution of electron transport compelxes in bacterial membranes, and its influence on electron transfer pathways, Publisher: ELSEVIER SCIENCE BV, Pages: S149-S150, ISSN: 0005-2728
Liu L-N, Bryan SJ, Huang F, et al., 2012, Control of electron transport routes through redox-regulated redistribution of respiratory complexes, 17th European Bioenergetics Conference, Publisher: ELSEVIER, Pages: S139-S139, ISSN: 0005-2728
Boehm M, Yu J, Krynicka V, et al., 2012, Subunit Organization of a <i>Synechocystis</i> Hetero-Oligomeric Thylakoid FtsH Complex Involved in Photosystem II Repair, PLANT CELL, Vol: 24, Pages: 3669-3683, ISSN: 1040-4651
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- Citations: 46
Michoux F, Nixon PJ, 2012, Towards the sustainable <i>in</i>-<i>vitro</i> production of plant derived anticancer compounds, International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF, Publisher: GEORG THIEME VERLAG KG, Pages: 1067-1068, ISSN: 0032-0943
Ahmad N, Michoux F, Nixon PJ, 2012, Investigating the production of foreign membrane proteins in tobacco chloroplasts: expression of an algal plastid terminal oxidase, PLoS One, Vol: 7, Pages: 1-10, ISSN: 1932-6203
Chloroplast transformation provides an inexpensive, easily scalable production platform for expression of recombinant proteins in plants. However, this technology has been largely limited to the production of soluble proteins. Here we have tested the ability of tobacco chloroplasts to express a membrane protein, namely plastid terminal oxidase 1 from the green alga Chlamydomonas reinhardtii (Cr-PTOX1), which is predicted to function as a plastoquinol oxidase. A homoplastomic plant containing a codon-optimised version of the nuclear gene encoding PTOX1, driven by the 16S rRNA promoter and 5′UTR of gene 10 from phage T7, was generated using a particle delivery system. Accumulation of Cr-PTOX1 was shown by immunoblotting and expression in an enzymatically active form was confirmed by using chlorophyll fluorescence to measure changes in the redox state of the plastoquinone pool in leaves. Growth of Cr-PTOX1 expressing plants was, however, more sensitive to high light than WT. Overall our results confirm the feasibility of using plastid transformation as a means of expressing foreign membrane proteins in the chloroplast.
Romero E, Diner BA, Nixon PJ, et al., 2012, Mixed Exciton-Charge-Transfer States in Photosystem II: Stark Spectroscopy on Site-Directed Mutants, BIOPHYSICAL JOURNAL, Vol: 103, Pages: 185-194, ISSN: 0006-3495
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- Citations: 51
Liu L-N, Bryan SJ, Huang F, et al., 2012, Control of electron transport routes through redox-regulated redistribution of respiratory complexes, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 109, Pages: 11431-11436, ISSN: 0027-8424
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- Citations: 71
Komenda J, Sobotka R, Nixon PJ, 2012, Assembling and maintaining the Photosystem II complex in chloroplasts and cyanobacteria, CURRENT OPINION IN PLANT BIOLOGY, Vol: 15, Pages: 245-251, ISSN: 1369-5266
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- Citations: 201
Ahmad N, Michoux F, McCarthy J, et al., 2012, Expression of the affinity tags, glutathione-S-transferase and maltose-binding protein, in tobacco chloroplasts, PLANTA, Vol: 235, Pages: 863-871, ISSN: 0032-0935
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- Citations: 19
Michoux F, Takasaka K, Boehm M, et al., 2012, Crystal structure of the Psb27 assembly factor at 1.6: implications for binding to Photosystem II, PHOTOSYNTHESIS RESEARCH, Vol: 110, Pages: 169-175, ISSN: 0166-8595
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- Citations: 23
Komenda J, Knoppova J, Kopecna J, et al., 2012, The Psb27 Assembly Factor Binds to the CP43 Complex of Photosystem II in the Cyanobacterium <i>Synechocystis</i> sp PCC 6803, PLANT PHYSIOLOGY, Vol: 158, Pages: 476-486, ISSN: 0032-0889
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- Citations: 92
Komenda J, Michoux F, Nixon PJ, 2012, Keeping the green world alive:the repair cycle of photosystem II, Self-Healing at the nanoscale, Editors: Amendola, Meneghetti, Publisher: Taylor & Francis Group, LLC, Pages: 3-22, ISBN: 978-1-4398-5473-0
Nguyen AV, Toepel J, Burgess S, et al., 2011, Time-course global expression profiles of Chlamydomonas reinhardtii during photo-biological H₂ production., PLOS One, Vol: 6, ISSN: 1932-6203
We used a microarray study in order to compare the time course expression profiles of two Chlamydomonas reinhardtii strains, namely the high H₂ producing mutant stm6glc4 and its parental WT strain during H₂ production induced by sulfur starvation. Major cellular reorganizations in photosynthetic apparatus, sulfur and carbon metabolism upon H₂ production were confirmed as common to both strains. More importantly, our results pointed out factors which lead to the higher H₂ production in the mutant including a higher starch accumulation in the aerobic phase and a lower competition between the H₂ase pathway and alternative electron sinks within the H₂ production phase. Key candidate genes of interest with differential expression pattern include LHCSR3, essential for efficient energy quenching (qE). The reduced LHCSR3 protein expression in mutant stm6glc4 could be closely related to the high-light sensitive phenotype. H₂ measurements carried out with the LHCSR3 knock-out mutant npq4 however clearly demonstrated that a complete loss of this protein has almost no impact on H₂ yields under moderate light conditions. The nuclear gene disrupted in the high H₂ producing mutant stm6glc4 encodes for the mitochondrial transcription termination factor (mTERF) MOC1, whose expression strongly increases during -S-induced H₂ production in WT strains. Studies under phototrophic high-light conditions demonstrated that the presence of functional MOC1 is a prerequisite for proper LHCSR3 expression. Furthermore knock-down of MOC1 in a WT strain was shown to improve the total H₂ yield significantly suggesting that this strategy could be applied to further enhance H₂ production in other strains already displaying a high H₂ production capacity. By combining our array data with previously published metabolomics data we can now explain some of the phenotypic characteristics which lead to an elevated H₂ production in stm6glc4.
Michoux F, Nixon PJ, 2011, Towards the sustainable and continuous <i>in</i>-<i>vitro</i> production of active pharmaceutical ingredients from medicinal plants, 59th International Congress and Annual Meeting of the Society-for-Medicinal-Plant-and-Natural-Product-Research, Publisher: GEORG THIEME VERLAG KG, Pages: 1281-1281, ISSN: 0032-0943
Michoux F, Ahmad N, McCarthy J, et al., 2011, Contained and high-level production of recombinant protein in plant chloroplasts using a temporary immersion bioreactor, PLANT BIOTECHNOLOGY JOURNAL, Vol: 9, Pages: 575-584, ISSN: 1467-7644
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- Citations: 27
Boehm M, Romero E, Reisinger V, et al., 2011, Investigating the Early Stages of Photosystem II Assembly in Synechocystis sp PCC 6803 <i>ISOLATION OF CP47 AND CP43 COMPLEXES</i>, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 286, Pages: 14812-14819
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- Citations: 76
Michoux F, McCarthy J, Nixon PJ, 2011, Method for producing leafy biomass in culture, WO/2011/030083
Battchikova N, Aro E-M, Nixon PJ, 2011, Structure and physiological function of NDH-1 complexes in cyanobacteria, The Bioenergetic processes of cyanobacteria, Editors: Peschek, Renger, Obinger, Dordrecht, The Netherlands, Publisher: Springer, Pages: 445-467
Burgess SJ, Tamburic B, Zemichael F, et al., 2011, Solar-Driven Hydrogen Production in Green Algae, ADVANCES IN APPLIED MICROBIOLOGY, VOL 75, Vol: 75, Pages: 71-110, ISSN: 0065-2164
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- Citations: 34
Michoux F, Takasaka K, Boehm M, et al., 2010, Structure of CyanoP at 2.8 Å: Implications for the Evolution and Function of the PsbP Subunit of Photosystem II, BIOCHEMISTRY, Vol: 49, Pages: 7411-7413, ISSN: 0006-2960
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- Citations: 43
Nixon PJ, Michoux F, Yu J, et al., 2010, Recent advances in understanding the assembly and repair of photosystem II, ANNALS OF BOTANY, Vol: 106, Pages: 1-16, ISSN: 0305-7364
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- Citations: 405
Komenda J, Knoppova J, Krynicka V, et al., 2010, Role of FtsH2 in the repair of Photosystem II in mutants of the cyanobacterium <i>Synechocystis</i> PCC 6803 with impaired assembly or stability of the CaMn<sub>4</sub> cluster, BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Vol: 1797, Pages: 566-575, ISSN: 0005-2728
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- Citations: 37
Burgess SJ, Nixon PJ, 2010, Investigating the Link between Fermentative Metabolism and Hydrogen Production in the Unicellular Green Alga Chlamydomonas Reinhardtii, Pages: 111-116
In the model green alga Chlamydomonas reinhardtii, the electrons required for hydrogen production can come from both the biophotolysis of water and from the fermentation of carbohydrate reserves. Anoxia leads to the activation of several fermentative pathways, which produce a number of end products including formic, malic and acetic acid along with ethanol, carbon dioxide and hydrogen. It has been proposed that by switching off competing fermentative pathways hydrogen production can be increased. Therefore the aim of this study was to devise an experimental strategy to down-regulate the expression of enzymes thought to control C. reinhardtii's fermentative metabolism. We demonstrate here that it is possible to use artificial microRNA (amiRNA) technology to generate knock-down mutants with reduced expression of pyruvate formate lyase (PFL1), a key fermentative enzyme in C. reinhardtii. This work opens up new possibilities to improve hydrogen yields through metabolic engineering.
Tamburic B, Burgess S, Nixon PJ, et al., 2010, An Experimental Study of the Growth and Hydrogen Production of C. Reinhardtii, Pages: 47-52
Some unicellular green algae, such as C. reinhardtii, have the ability to photosynthetically produce molecular hydrogen under anaerobic conditions. They offer a biological route to renewable, carbon-neutral hydrogen production from two of nature’s most plentiful resources – sunlight and water. This process provides the additional benefit of carbon dioxide sequestration and the option of deriving valuable products from algal biomass. The growth of dense and healthy algal biomass is a prerequisite for efficient hydrogen production. This study investigates the growth of C. reinhardtii under different cyclic light regimes and at various continuous light intensities. Algal growth is characterised in terms of the cell count, chlorophyll content and optical density of the culture. The consumption of critical nutrients such as acetate and sulphate is measured by chromatography techniques. C. reinhardtii wild-type CC-124 strain is analysed in a 3 litre tubular flow photobioreactor featuring a large surface-to-volume ratio and excellent light penetration through the culture. Key parameters of the hydrogen production process are continuously monitored and controlled; these include pH, pO2, optical density, temperature, agitation and light intensity. Gas phase hydrogen production is determined by mass spectrometry.
Boehm M, Nield J, Zhang P, et al., 2009, Structural and Mutational Analysis of Band 7 Proteins in the Cyanobacterium <i>Synechocystis</i> sp Strain PCC 6803, JOURNAL OF BACTERIOLOGY, Vol: 191, Pages: 6425-6435, ISSN: 0021-9193
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- Citations: 37
Michoux F, Nixon P, Mccarthy J, 2009, Contained and high-level production of recombinant protein in plant chloroplasts, Publisher: ELSEVIER SCIENCE BV, Pages: S294-S294, ISSN: 1871-6784
Komenda J, Nickelsen J, Tichy M, et al., 2008, The cyanobacterial homologue of HCF136/YCF48 is a component of an early photosystem II assembly complex and is important for both the efficient assembly and repair of photosystem II in Synechocystis sp PCC 6803, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 283, Pages: 22390-22399, ISSN: 0021-9258
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- Citations: 113
Schlodder E, Coleman WJ, Nixon PJ, et al., 2008, Site-directed mutations at D1-His198 and D1-Thr179 of photosystem II in <i>Synechocystis</i> sp PCC 6803:: deciphering the spectral properties of the PSII reaction centre, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 363, Pages: 1197-1202, ISSN: 0962-8436
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- Citations: 20
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