Publications
173 results found
P J Nixon, C W Mullineaux, 2001, Regulation of photosynthetic electron transport, Regulatory aspects of photosynthesis, Editors: Andersson, Aro, Dordrecht, The Netherlands, Publisher: Kluwer Academic Publishers, Pages: 533-555
Stewart DH, Nixon PJ, Diner BA, et al., 2000, Assignment of the Q<sub><i>y</i></sub> absorbance bands of photosystem II chromophores by low-temperature optical spectroscopy of wild-type and mutant reaction centers, BIOCHEMISTRY, Vol: 39, Pages: 14583-14594, ISSN: 0006-2960
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- Citations: 34
Nixon PJ, 2000, Chlororespiration, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 355, Pages: 1541-1547, ISSN: 0962-8436
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- Citations: 93
Campbell KA, Force DA, Nixon PJ, et al., 2000, Dual-mode EPR detects the initial intermediate in photoassembly of the photosystem II mn cluster: The influence of amino acid residue 170 of the D1 polypeptide on Mn coordination, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 122, Pages: 3754-3761, ISSN: 0002-7863
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- Citations: 121
Komenda J, Hassan HAG, Diner BA, et al., 2000, Degradation of the Photosystem II D1 and D2 proteins in different strains of the cyanobacterium <i>Synechocystis</i> PCC 6803 varying with respect to the type and level of <i>psbA</i> transcript, PLANT MOLECULAR BIOLOGY, Vol: 42, Pages: 635-645, ISSN: 0167-4412
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- Citations: 45
Kruse O, Nixon PJ, Schmid GH, et al., 1999, Isolation of state transition mutants of <i>Chlamydomonas reinhardtii</i> by fluorescence video imaging, PHOTOSYNTHESIS RESEARCH, Vol: 61, Pages: 43-51, ISSN: 0166-8595
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- Citations: 42
Alizadeh S, Morais F, Barber J, et al., 1999, Isotopic labelling of the polypeptide subunits of the isolated photosystem II reaction-centre complex of <i>Chlamydomonas reinhardtii</i> suggests an αβ heterodimeric structure for cytochrome <i>b</i>-559, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, Vol: 48, Pages: 148-153, ISSN: 1011-1344
Nixon PJ, Maliga P, 1999, Reply... Chlororespiration: only half a story, TRENDS IN PLANT SCIENCE, Vol: 4, Pages: 51-51, ISSN: 1360-1385
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- Citations: 5
Andronis C, Merry SAP, Durrant JR, et al., 1999, Mutation of the Chlamydomonas reinhardtii analogue of residue M210 of the Rhodobacter sphaeroides reaction center slows primary electron transfer in Photosystem II, Photosynthesis Research, Vol: 62, Pages: 205-217, ISSN: 0166-8595
Merry SAP, Nixon PJ, Barter LMC, et al., 1998, Modulation of Quantum Yield of Primary Radical Pair Formation in Photosystem II by Site-Directed Mutagenesis Affecting Radical Cations and Anions, Biochemistry, Vol: 37, Pages: 17439-17447, ISSN: 0006-2960
Morais F, Barber J, Nixon PJ, 1998, The chloroplast-encoded α subunit of cytochrome <i>b</i>-559 is required for assembly of the photosystem two complex in both the light and the dark in <i>Chlamydomonas reinhardtii</i>, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 273, Pages: 29315-29320, ISSN: 0021-9258
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- Citations: 67
Maliga P, Nixon PJ, 1998, Judging the homoplastomic state of plastid transformants, TRENDS IN PLANT SCIENCE, Vol: 3, Pages: 376-377, ISSN: 1360-1385
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- Citations: 26
Sazanov LA, Burrows PA, Nixon PJ, 1998, The chloroplast Ndh complex mediates the dark reduction of the plastoquinone pool in response to heat stress in tobacco leaves, FEBS LETTERS, Vol: 429, Pages: 115-118, ISSN: 0014-5793
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- Citations: 106
Andronis C, Kruse O, Deák Z, et al., 1998, Mutation of residue threonine-2 of the D2 polypeptide and its effect on photosystem II function in <i>Chlamydomonas reinhardtii</i>, PLANT PHYSIOLOGY, Vol: 117, Pages: 515-524, ISSN: 0032-0889
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- Citations: 23
Burrows PA, Sazanov LA, Svab Z, et al., 1998, Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid <i>ndh</i> genes, EMBO JOURNAL, Vol: 17, Pages: 868-876, ISSN: 0261-4189
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- Citations: 401
Sazanov LA, Burrows PA, Nixon PJ, 1998, The plastid <i>ndh</i> genes code for an NADH-specific dehydrogenase:: Isolation of a complex I analogue from pea thylakoid membranes, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 95, Pages: 1319-1324, ISSN: 0027-8424
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- Citations: 168
Durrant JR, Nixon PJ, Barber J, et al., 1998, Identification of chlorophyll anion states during charge separation in mutant photosystem II reaction centres., XIth International Congress on Photosynthesis - Mechanisms and Effects, Publisher: SPRINGER, Pages: 1041-1044
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- Citations: 2
Morais F, Barber J, Nixon PJ, 1998, Cytochrome <i>b</i>-559 protects photosystem two in the light and the dark in <i>Chlamydomonas reinhardtii</i>, XIth International Congress on Photosynthesis - Mechanisms and Effects, Publisher: SPRINGER, Pages: 1109-1112
Merry SAP, Nixon PJ, Barter LMC, et al., 1998, Modulation of quantum yield of primary radical pair formation in photosystem II by site-directed mutagenesis affecting radical cations and anions, Biochemistry, Vol: 37, Pages: 17439-17447, ISSN: 0006-2960
Dalla Chiesa M, Friso G, Deák Z, et al., 1997, Reduced turnover of the D1 polypeptide and photoactivation of electron transfer in novel herbicide resistant mutants of Synechocystis sp. PCC 6803., Eur J Biochem, Vol: 248, Pages: 731-740, ISSN: 0014-2956
Two missense mutants, A263P and S264P, and a deletion mutant des-Ala263, Ser264, have been constructed in the D1 protein of the cyanobacterium Synechocystis sp PCC 6803. All were expected to induce a significant conformational change in the QB-binding region of photosystem II (PSII). Although the des-Ala263, Ser264-D1 mutant accumulated some D1 protein in the thylakoid membrane it was unable to grow photoautotrophically or evolve oxygen. Thermoluminescence and chlorophyll fluorescence studies confirmed that this deletion mutant did not show any functional PSII activity. In contrast, [S264P]D1 was able to grow photoautotrophically and give light-saturated rates of oxygen evolution at 60% of the rate of the wild-type control strain, TC31. The A263P missense mutant was also able to evolve oxygen at 50% of TC31 rates although it did not readily grow photoautotrophically. Thermoluminescence, flash oxygen yield and chlorophyll fluorescence measurements indicated that in both missense mutants electron transfer from QA to QB was significantly impaired in dark adapted cells. However, QA to QB electron transfer could be photoactivated in the mutants by background illumination. Both the A263P and S264P mutants also showed an increase in resistance to the s-triazine family of herbicides although this feature did not hold for the phenolic herbicide, ioxynil. Of particular interest was that the two missense mutants, especially S264P, possessed a slower rate of turnover of the D1 protein compared with TC31 and in vivo contained detectable levels of a 41-kDa adduct consisting of D1 and the alpha subunit of cytochrome b559. When protein synthesis was blocked by the addition of lincomycin, D1 degradation was again slower in S264P than TC31. The results are discussed in terms of structural changes in the QB-binding region which affect herbicide and plastoquinone binding and perturb the normal regulatory factors that control the degradation of the D1 protein and its synchronisation wit
DallaChiesa M, Deak Z, Vass I, et al., 1996, The lumenal loop connecting transmembrane helices I and II of the D1 polypeptide is important for assembly of the photosystem two complex, PHOTOSYNTHESIS RESEARCH, Vol: 50, Pages: 79-91, ISSN: 0166-8595
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- Citations: 4
Sazanov LA, Burrows P, Nixon PJ, 1996, Detection and characterization of a complex I-like NADH-specific dehydrogenase from pea thylakoids, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 24, Pages: 739-743, ISSN: 0300-5127
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- Citations: 43
James EK, Iannetta PPM, Nixon PJ, et al., 1996, Photosystem II and oxygen regulation in Sesbania rostrata stem nodules, PLANT CELL AND ENVIRONMENT, Vol: 19, Pages: 895-910, ISSN: 0140-7791
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- Citations: 27
Giorgi LB, Nixon PJ, Merry SA, et al., 1996, Comparison of primary charge separation in the photosystem II reaction center complex isolated from wild-type and D1-130 mutants of the cyanobacterium Synechocystis PCC 6803., J Biol Chem, Vol: 271, Pages: 2093-2101, ISSN: 0021-9258
We compare primary charge separation in a photosystem II reaction center preparation isolated from a wild-type (WT) control strain of the cyanobacterium Synechocystis sp. PCC 6803 and from two site-directed mutants of Synechocystis in which residue 130 of the D1 polypeptide has been changed from a glutamine to either a glutamate (mutant D1-Gln130Glu), as in higher plant sequences, or a leucine residue (mutant D1-Gln130Leu). The D1-130 residue is thought to be close to the pheophytin electron acceptor. We show that, when P680 is photoselectively excited, the primary radical pair state P680+Ph- is formed with a time constant of 20-30 ps in the WT and both mutants; this time constant is very similar to that observed in Pisum sativum (a higher plant). We also show that a change in the residue at position D1-130 causes a shift in the peak of the pheophytin Qx-band. Nanosecond and picosecond transient absorption measurements indicate that the quantum yield of radical pair formation (phi RP), associated with the 20-30-ps component, is affected by the identify of the D1-130 residue. We find that, for the isolated photosystem II reaction center particle, phi RP higher plant > phi RP D1-Gln130Glu mutant > phi RP WT > phi RP D1-Gln130Leu mutant. Furthermore, the spectroscopic and quantum yield differences we observe between the WT Synechocystis and higher plant photosystem II, seem to be reversed by mutating the D1-130 ligand so that it is the same as in higher plants. This result is consistent with the previously observed natural regulation of quantum yield in Synechococcus PS II by particular changes in the D1 polypeptide amino acid sequence (Clark, A.K., Hurry, V. M., Gustafsson, P. and Oquist, G. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 11985-11989).
P Nixon, C Jansson, 1996, Cyanobacterial transformation and gene regulation, Molecular Genetics of Photosynthesis, Editors: Andersson, Salter, Barber, Oxford, UK, Publisher: Oxford University Press, Pages: 197-224
Giorgi LB, Nixon PJ, Merry SAP, et al., 1996, Comparison of PS II primary photochemistry in higher plant, synechocystis and synechocystis mutants, Current Research in Photosynthesis, Publisher: Kluwer Academic Publishers
NIXON PJ, KOMENDA J, BARBER J, et al., 1995, DELETION OF THE PEST-LIKE REGION OF PHOTOSYSTEM-2 MODIFIES THE Q(B)-BINDING POCKET BUT DOES NOT PREVENT RAPID TURNOVER OF D1, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 270, Pages: 14919-14927, ISSN: 0021-9258
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- Citations: 62
ALIZADEH S, NIXON PJ, TELFER A, et al., 1995, ISOLATION AND CHARACTERIZATION OF THE PHOTOSYSTEM 2 REACTION-CENTER COMPLEX FROM A DOUBLE MUTANT OF CHLAMYDOMONAS-REINHARDTII, PHOTOSYNTHESIS RESEARCH, Vol: 43, Pages: 165-171, ISSN: 0166-8595
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- Citations: 12
Vermaas W, Madsen C, Yu J, et al., 1995, Turnover of the D1 protein and of photosystem II in a Synechocystis 6803 mutant lacking TyrZ, Photosynth. Res., Vol: 45, Pages: 99-104
Morais F, Barber J, Nixon PJ, 1995, Insertional inactivation of the chloroplast psbE gene in the green alga Chlamydomonas reinhardtii., Xth International Photosynthesis Congress, Publisher: KLUWER ACADEMIC PUBL, Pages: 791-794
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