Imperial College London
Alternate

ProfessorPeterNixon

Faculty of Natural Sciences, Department of Life Sciences

Professor of Biochemistry
 
 
 
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Contact

 

+44 (0)20 7594 5269p.nixon

 
 
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Location

 

705Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bečková:2016:10.1016/j.molp.2016.08.001,
author = {Beková, M and Gardian, Z and Yu, J and Konik, P and Nixon, PJ and Komenda, J},
doi = {10.1016/j.molp.2016.08.001},
journal = {Molecular Plant},
pages = {62--72},
title = {Association of Psb28 and Psb27 proteins with PSII-PSI supercomplexes upon exposure of Synechocystis sp. PCC 6803 to high light},
url = {http://dx.doi.org/10.1016/j.molp.2016.08.001},
volume = {10},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Formation of the multi-subunit oxygen-evolving Photosystem II (PSII) complex involvesa number of auxiliary protein factors. In this study we compared the location and possiblefunction of two homologous PSII assembly factors, Psb28-1 and Psb28-2, from thecyanobacterium Synechocystis sp. PCC 6803. We show that FLAG-tagged Psb28-2 ispresent in both the monomeric PSII core complex and a PSII core complex lacking theinner antenna CP43 (RC47) whereas Psb28-1 preferentially binds to RC47. When cellsare exposed to increased irradiance, both tagged Psb28 proteins now associate witholigomeric forms of PSII and with PSII-PSI supercomplexes composed of trimericPhotosystem I (PSI) and two PSII monomers as deduced from negative stain electronmicroscopy. The presence of the Psb27 accessory protein in these complexes suggests theinvolvement of PSI in PSII biogenesis, possibly by photoprotecting PSII through energyspillover. Under standard cultivation conditions the distribution of PSII complexes issimilar in WT and each of the single psb28 null mutants except for loss of RC47 in theabsence of Psb28-1. In comparison with WT, growth of mutants lacking Psb28-1 andPsb27, but not Psb28-2, was retarded under high-light and, especially, intermittent highlight-darkconditions, emphasizing the physiological importance of PSII assembly factorsfor light acclimation.
AU  - Beková,M
AU  - Gardian,Z
AU  - Yu,J
AU  - Konik,P
AU  - Nixon,PJ
AU  - Komenda,J
DO  - 10.1016/j.molp.2016.08.001
EP  - 72
PY  - 2016///
SN  - 1752-9867
SP  - 62
TI  - Association of Psb28 and Psb27 proteins with PSII-PSI supercomplexes upon exposure of Synechocystis sp. PCC 6803 to high light
T2  - Molecular Plant
UR  - http://dx.doi.org/10.1016/j.molp.2016.08.001
UR  - http://hdl.handle.net/10044/1/38672
VL  - 10
ER  -
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