Imperial College London

DrPhilipPratt

Faculty of MedicineDepartment of Surgery & Cancer

 
 
 
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Contact

 

+44 (0)20 3312 5525p.pratt Website

 
 
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Location

 

005Paterson WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tarunina:2016:10.1089/scd.2016.0216,
author = {Tarunina, M and Hernandez, D and Kronsteiner-Dobramysl, B and Pratt, P and Watson, T and Hua, P and Gullo, F and Van, der Garde M and Zhang, Y and Hook, L and Choo, Y and Watt, S},
doi = {10.1089/scd.2016.0216},
journal = {Stem Cells and Development},
title = {A novel high throughput screening platform reveals an optimised cytokine formulation for human hematopoietic progenitor cell expansion},
url = {http://dx.doi.org/10.1089/scd.2016.0216},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The main limitations of hematopoietic cord blood (CB) transplantation, viz. low cell dosage and delayed reconstitution, can be overcome by ex-vivo expansion. CB expansion under conventional culture causes the rapid cell differentiation and depletion of hematopoietic stem/progenitor cells (HSPC) responsible for engraftment. Here, we use combinatorial cell culture technology (CombiCult®) to identify media formulations that promote CD133+ CB HSPC proliferation while maintaining their phenotypic characteristics. We employed second generation CombiCult® screens that use electro-spraying technology to encapsulate CB cells in alginate beads. Our results suggest that, not only the combination, but also the order of addition of individual components has a profound influence on expansion of specific HSPC populations. Top protocols identified by the CombiCult® screen were used to culture human CD133+ CB HSPCs on nanofiber scaffolds and validate the expansion of the phenotypically defined CD34+CD38lo/-CD45RA-CD90+CD49f+ population of hematopoietic stem cells and their differentiation into defined progeny.
AU - Tarunina,M
AU - Hernandez,D
AU - Kronsteiner-Dobramysl,B
AU - Pratt,P
AU - Watson,T
AU - Hua,P
AU - Gullo,F
AU - Van,der Garde M
AU - Zhang,Y
AU - Hook,L
AU - Choo,Y
AU - Watt,S
DO - 10.1089/scd.2016.0216
PY - 2016///
SN - 1557-8534
TI - A novel high throughput screening platform reveals an optimised cytokine formulation for human hematopoietic progenitor cell expansion
T2 - Stem Cells and Development
UR - http://dx.doi.org/10.1089/scd.2016.0216
ER -