Summary
Dr Petr Vikhorev is currently working as a research associate at the Department of Bioengineering. He joined the department in 2021 where he developed software for custom high-speed laser scanning microscopy coupled with interferometry and photonic stimulation to study auditory hair cells.
He has been working at Imperial College London since 2009. Firstly, he joined the Faculty of Medicine as a research associate working in the lab of Professor Michael Ferenczi. Later, he worked with Professor Steve Marston and in 2017 he started an independent research career as a research fellow at the National Heart and Lung Institute. In 2017-2020, he was the principal investigator of the BHF research project grant “Effect of mutations associated with dilated and hypertrophic cardiomyopathies on myofibril mechanical properties and contractility”. His research interests focused on the mechanisms and regulation of muscle contraction.
He has a broad range of biophysics expertise including single myofibril force measurements, polarised fluorescence fluorometry, in vitro motility assay technique, analytical and preparative biochemistry, computer control of instrumentation and creating LabVIEW software. He built the apparatus and wrote the software for the measurement and analysis of myofibril contractile dynamics and used this technique in the study of cardiomyopathy using an established collection of human heart tissue samples. He used genomics, transcriptomics, and proteomics to understand the biological mechanisms of heart failure. Integrating experimental data with mathematical modelling and computer simulations, he led multidisciplinary translational research to find better strategies for the treatment of cardiomyopathies.
Selected Publications
Journal Articles
Vikhorev P, Vikhoreva N, Yeung W, et al. , 2022, Titin-truncating mutations associated with dilated cardiomyopathy alter length-dependent activation and its modulation via phosphorylation, Cardiovascular Research, Vol:118, ISSN:0008-6363, Pages:241-253
Tucholski T, Cai W, Gregorich ZR, et al. , 2020, Distinct hypertrophic cardiomyopathy genotypes result in convergent sarcomeric proteoform profiles revealed by top-down proteomics, Proceedings of the National Academy of Sciences of the United States of America, Vol:117, ISSN:0027-8424, Pages:24691-24700
Vikhorev P, Vikhoreva N, 2018, Cardiomyopathies and related changes in contractility of human heart muscle, International Journal of Molecular Sciences, Vol:19, ISSN:1422-0067
Vikhorev, Smoktunowicz N, Munster A, et al. , 2017, Abnormal contractility in human heart myofibrils from patients with dilated cardiomyopathy due to mutations in TTN and contractile protein genes., Scientific Reports, Vol:7, ISSN:2045-2322
Vikhorev P, Marston S, Ferenczi M, 2016, Instrumentation to Study Myofibril Mechanics from Static to Artificial Simulations of Cardiac Cycle, Methodsx, Vol:3, ISSN:2215-0161, Pages:156-170
Papadaki M, Vikhorev PG, Marston SB, et al. , 2015, Uncoupling of myofilament Ca2+-sensitivity from troponin I phosphorylation by mutations can be reversed by Epigallocatechin-3-Gallate., Cardiovascular Research, Vol:108, ISSN:1755-3245, Pages:99-110
Vikhorev PG, Song W, Wilkinson R, et al. , 2014, The dilated cardiomyopathy-causing mutation ACTC E361G in cardiac muscle myofibrils specifically abolishes modulation of Ca2+ regulation by phosphorylation of Troponin I, Biophysical Journal, Vol:107, ISSN:1542-0086, Pages:2369-2380
Song W, Vikhorev PG, Kashyap MN, et al. , 2013, Mechanical and energetic properties of papillary muscle from <i>ACTC</i> E99K transgenic mouse models of hypertrophic cardiomyopathy, American Journal of Physiology - Heart and Circulatory Physiology, Vol:304, ISSN:0363-6135, Pages:H1513-H1524