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@article{Chadeau:2017:10.1016/j.envint.2017.08.006,
author = {Chadeau, M and Plusquin, M and Guida, F and Polidoro, S and Vermeulen, R and Raaschou-Nielsen, O and Campanella, G and Hoek, G and Kyrtopoulos, SA and Georgiadis, P and Naccarati, A and Sacerdote, C and Krogh, V and Bueno-de-Mesquita, B and Verschuren, M and Sayols-Baixeras, S and Panni, T and Peters, A and Hebels, D and Kleinjans, J and Vineis, P},
doi = {10.1016/j.envint.2017.08.006},
journal = {Environment International},
pages = {127--136},
title = {DNA methylation and exposure to ambient air pollution in two prospective cohorts},
url = {http://dx.doi.org/10.1016/j.envint.2017.08.006},
volume = {108},
year = {2017}
}

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TY  - JOUR
AB  - Long-term exposure to air pollution has been associated with several adverse health effects including cardiovascular, respiratory diseases and cancers. However, underlying molecular alterations remain to be further investigated. The aim of this study is to investigate the effects of long-term exposure to air pollutants on (a) average DNA methylation at functional regions and, (b) individual differentially methylated CpG sites. An assumption is that omic measurements, including the methylome, are more sensitive to low doses than hard health outcomes.This study included blood-derived DNA methylation (Illumina-HM450 methylation) for 454 Italian and 159 Dutch participants from the European Prospective Investigation into Cancer and Nutrition (EPIC). Long-term air pollution exposure levels, including NO2, NOx, PM2.5, PMcoarse, PM10, PM2.5 absorbance (soot) were estimated using models developed within the ESCAPE project, and back-extrapolated to the time of sampling when possible. We meta-analysed the associations between the air pollutants and global DNA methylation, methylation in functional regions and epigenome-wide methylation. CpG sites found differentially methylated with air pollution were further investigated for functional interpretation in an independent population (EnviroGenoMarkers project), where (N = 613) participants had both methylation and gene expression data available.Exposure to NO2 was associated with a significant global somatic hypomethylation (p-value = 0.014). Hypomethylation of CpG island's shores and shelves and gene bodies was significantly associated with higher exposures to NO2 and NOx. Meta-analysing the epigenome-wide findings of the 2 cohorts did not show genome-wide significant associations at single CpG site level. However, several significant CpG were found if the analyses were separated by countries. By regressing gene expression levels against methylation levels of the exposure-related CpG sites, we identified several significant CpG-
AU  - Chadeau,M
AU  - Plusquin,M
AU  - Guida,F
AU  - Polidoro,S
AU  - Vermeulen,R
AU  - Raaschou-Nielsen,O
AU  - Campanella,G
AU  - Hoek,G
AU  - Kyrtopoulos,SA
AU  - Georgiadis,P
AU  - Naccarati,A
AU  - Sacerdote,C
AU  - Krogh,V
AU  - Bueno-de-Mesquita,B
AU  - Verschuren,M
AU  - Sayols-Baixeras,S
AU  - Panni,T
AU  - Peters,A
AU  - Hebels,D
AU  - Kleinjans,J
AU  - Vineis,P
DO  - 10.1016/j.envint.2017.08.006
EP  - 136
PY  - 2017///
SN  - 0160-4120
SP  - 127
TI  - DNA methylation and exposure to ambient air pollution in two prospective cohorts
T2  - Environment International
UR  - http://dx.doi.org/10.1016/j.envint.2017.08.006
UR  - http://hdl.handle.net/10044/1/51526
VL  - 108
ER  -

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