Imperial College London
Alternate

ProfessorPetraHajkova

Faculty of Medicine, Institute of Clinical Sciences

Professor of Developmental Epigenetics
 
 
 
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Contact

 

+44 (0)20 7594 6754petra.hajkova Website

 
 
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Location

 

5.11CLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tang:2006:nar/gnj009,
author = {Tang, FC and Hajkova, P and Barton, SC and Lao, KQ and Surani, MA},
doi = {nar/gnj009},
journal = {Nucleic Acids Research},
pages = {1--7},
title = {MicroRNA expression profiling of single whole embryonic stem cells},
url = {http://dx.doi.org/10.1093/nar/gnj009},
volume = {34},
year = {2006}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - MicroRNAs (miRNAs) are a class of 17–25 nt non-coding RNAs that have been shown to have critical functions in a wide variety of biological processes during development. Recently developed miRNA microarray techniques have helped to accelerate research on miRNAs. However, in some instances there is only a limited amount of material available for analysis, which requires more sensitive techniques that can preferably work on single cells. Here we demonstrate that it is possible to analyse miRNA in single cells by using a real-time PCR-based 220-plex miRNA expression profiling method. Development of this technique will greatly facilitate miRNA-related research on cells, such as the founder population of primordial germ cells where rapid and dynamic changes occur in a few cells, and for analysing heterogeneous population of cells. In these and similar cases, our method of single cell analysis is critical for elucidating the diverse roles of miRNAs.
AU  - Tang,FC
AU  - Hajkova,P
AU  - Barton,SC
AU  - Lao,KQ
AU  - Surani,MA
DO  - nar/gnj009
EP  - 7
PY  - 2006///
SN  - 0305-1048
SP  - 1
TI  - MicroRNA expression profiling of single whole embryonic stem cells
T2  - Nucleic Acids Research
UR  - http://dx.doi.org/10.1093/nar/gnj009
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000235291300002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://academic.oup.com/nar/article/34/2/e9/2401734
UR  - http://hdl.handle.net/10044/1/85190
VL  - 34
ER  -
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