Imperial College London
Alternate

ProfessorPetraHajkova

Faculty of Medicine, Institute of Clinical Sciences

Professor of Developmental Epigenetics
 
 
 
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Contact

 

+44 (0)20 7594 6754petra.hajkova Website

 
 
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Location

 

5.11CLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Eguizabal:2016:10.1002/stem.2422,
author = {Eguizabal, C and Herrera, L and De, Onate L and Montserrat, N and Hajkova, P and Izpisua, Belmonte JC},
doi = {10.1002/stem.2422},
journal = {Stem Cells},
pages = {2418--2428},
title = {Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming},
url = {http://dx.doi.org/10.1002/stem.2422},
volume = {34},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Epigenetic reprogramming is a central process during mammalian germline development. Genome-wide DNA demethylation in primordial germ cells (PGCs) is a prerequisite for the erasure of epigenetic memory, preventing the transmission of epimutations to the next generation. Apart from DNA demethylation, germline reprogramming has been shown to entail reprogramming of histone marks and chromatin remodelling. Contrary to other animal models, there is limited information about the epigenetic dynamics during early germ cell development in humans. Here, we provide further characterization of the epigenetic configuration of the early human gonadal PGCs. We show that early gonadal human PGCs are DNA hypomethylated and their chromatin is characterized by low H3K9me2 and high H3K27me3 marks. Similarly to previous observations in mice, human gonadal PGCs undergo dynamic chromatin changes concomitant with the erasure of genomic imprints. Interestingly, and contrary to mouse early germ cells, expression of BLIMP1/PRDM1 persists in through all gestational stages in human gonadal PGCs and is associated with nuclear lysine-specific demethylase-1. Our work provides important additional information regarding the chromatin changes associated with human PGCs development between 6 and 13 weeks of gestation in male and female gonads.
AU  - Eguizabal,C
AU  - Herrera,L
AU  - De,Onate L
AU  - Montserrat,N
AU  - Hajkova,P
AU  - Izpisua,Belmonte JC
DO  - 10.1002/stem.2422
EP  - 2428
PY  - 2016///
SN  - 1066-5099
SP  - 2418
TI  - Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming
T2  - Stem Cells
UR  - http://dx.doi.org/10.1002/stem.2422
UR  - http://hdl.handle.net/10044/1/34388
VL  - 34
ER  -
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