Imperial College London
Alternate

ProfessorPetraHajkova

Faculty of Medicine, Institute of Clinical Sciences

Professor of Developmental Epigenetics
 
 
 
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Contact

 

+44 (0)20 7594 6754petra.hajkova Website

 
 
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Location

 

5.11CLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Benešová:2017:10.1002/mc.22638,
author = {Beneová, M and Trejbalová, K and Kuerová, D and Vernerová, Z and Hron, T and Szabó, A and Amouroux, R and Klézl, P and Hajkova, P and Hejnar, J},
doi = {10.1002/mc.22638},
journal = {Molecular Carcinogenesis},
pages = {1837--1850},
title = {Overexpression of TET dioxygenases in seminomas associates with low levels of DNA methylation and hydroxymethylation.},
url = {http://dx.doi.org/10.1002/mc.22638},
volume = {56},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Germ cell tumors and particularly seminomas reflect the epigenomic features of their parental primordial germ cells, including genomic DNA hypomethylation and expression of pluripotent cell markers. Because the DNA hypomethylation might be a result of TET dioxygenase activity, we examined expression of TET1-3 enzymes and the level of their product, 5-hydroxymethylcytosine, in a panel of histologically characterized seminomas and non-seminomatous germ cell tumors. Expression of TET dioxygenase mRNAs was quantified by real-time PCR. TET1 expression and the level of 5-hydroxymethylcytosine were examined immunohistochemically. Quantitative assessment of 5-methylcytosine and 5-hydroxymethylcytosine levels was done by the liquid chromatography-mass spectroscopy technique. We found highly increased expression of TET1 dioxygenase in most seminomas and strong TET1 staining in seminoma cells. Isocitrate dehydrogenase 1 and 2 mutations were not detected, suggesting the enzymatic activity of TET1. The levels of 5-methylcytosine and 5-hydroxymethylcytosine in seminomas were found decreased in comparison to non-seminomatous germ cell tumors and healthy testicular tissue. We propose that TET1 expression should be studied as a potential marker of seminomas and mixed germ cell tumors and we suggest that elevated expression of TET dioxygenase enzymes is associated with the maintenance of low DNA methylation levels in seminomas. This "anti-methylator" phenotype of seminomas is in contrast to the CpG island methylator phenotype observed in a fraction of tumors of various types.
AU  - Beneová,M
AU  - Trejbalová,K
AU  - Kuerová,D
AU  - Vernerová,Z
AU  - Hron,T
AU  - Szabó,A
AU  - Amouroux,R
AU  - Klézl,P
AU  - Hajkova,P
AU  - Hejnar,J
DO  - 10.1002/mc.22638
EP  - 1850
PY  - 2017///
SN  - 1098-2744
SP  - 1837
TI  - Overexpression of TET dioxygenases in seminomas associates with low levels of DNA methylation and hydroxymethylation.
T2  - Molecular Carcinogenesis
UR  - http://dx.doi.org/10.1002/mc.22638
UR  - http://hdl.handle.net/10044/1/45711
VL  - 56
ER  -
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