Imperial College London
Alternate

ProfessorPetraHajkova

Faculty of Medicine, Institute of Clinical Sciences

Professor of Developmental Epigenetics
 
 
 
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Contact

 

+44 (0)20 7594 6754petra.hajkova Website

 
 
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Location

 

5.11CLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ferry:2017:10.1016/j.molcel.2017.07.012,
author = {Ferry, L and Fournier, A and Tsusaka, T and Adelmant, G and Shimazu, T and Matano, S and Kirsh, O and Amouroux, R and Dohmae, N and Suzuki, T and Filion, GJ and Deng, W and de, Dieuleveult M and Fritsch, L and Kudithipudi, S and Jeltsch, A and Leonhardt, H and Hajkova, P and Marto, JA and Arita, K and Shinkai, Y and Defossez, P-A},
doi = {10.1016/j.molcel.2017.07.012},
journal = {Molecular Cell},
pages = {550--565.e5},
title = {Methylation of DNA ligase 1 by G9a/GLP recruits UHRF1 to replicating DNA and regulates DNA methylation},
url = {http://dx.doi.org/10.1016/j.molcel.2017.07.012},
volume = {67},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - DNA methylation is an essential epigenetic mark in mammals that has to be re-established after each round of DNA replication. The protein UHRF1 is essential for this process; it has been proposed that the protein targets newly replicated DNA by cooperatively binding hemi-methylated DNA and H3K9me2/3, but this model leaves a number of questions unanswered. Here, we present evidence for a direct recruitment of UHRF1 by the replication machinery via DNA ligase 1 (LIG1). A histone H3K9-like mimic within LIG1 is methylated by G9a and GLP and, compared with H3K9me2/3, more avidly binds UHRF1. Interaction with methylated LIG1 promotes the recruitment of UHRF1 to DNA replication sites and is required for DNA methylation maintenance. These results further elucidate the function of UHRF1, identify a non-histone target of G9a and GLP, and provide an example of a histone mimic that coordinates DNA replication and DNA methylation maintenance.
AU  - Ferry,L
AU  - Fournier,A
AU  - Tsusaka,T
AU  - Adelmant,G
AU  - Shimazu,T
AU  - Matano,S
AU  - Kirsh,O
AU  - Amouroux,R
AU  - Dohmae,N
AU  - Suzuki,T
AU  - Filion,GJ
AU  - Deng,W
AU  - de,Dieuleveult M
AU  - Fritsch,L
AU  - Kudithipudi,S
AU  - Jeltsch,A
AU  - Leonhardt,H
AU  - Hajkova,P
AU  - Marto,JA
AU  - Arita,K
AU  - Shinkai,Y
AU  - Defossez,P-A
DO  - 10.1016/j.molcel.2017.07.012
EP  - 565
PY  - 2017///
SN  - 1097-2765
SP  - 550
TI  - Methylation of DNA ligase 1 by G9a/GLP recruits UHRF1 to replicating DNA and regulates DNA methylation
T2  - Molecular Cell
UR  - http://dx.doi.org/10.1016/j.molcel.2017.07.012
UR  - https://www.ncbi.nlm.nih.gov/pubmed/28803780
UR  - https://www.sciencedirect.com/science/article/pii/S1097276517305063?via%3Dihub
UR  - http://hdl.handle.net/10044/1/50094
VL  - 67
ER  -
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