Imperial College London
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Dr Po-Heng (Henry) Lee

Faculty of Engineering, Department of Civil and Environmental Engineering

Senior Lecturer in Wastewater Engineering
 
 
 
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Contact

 

+44 (0)20 7594 5993po-heng.lee

 
 
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Location

 

310ASkempton BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ji:2019:10.1016/j.watres.2019.115039,
author = {Ji, X and Wu, Z and Sung, S and Lee, P-H},
doi = {10.1016/j.watres.2019.115039},
journal = {Water Res},
title = {Metagenomics and metatranscriptomics analyses reveal oxygen detoxification and mixotrophic potentials of an enriched anammox culture in a continuous stirred-tank reactor.},
url = {http://dx.doi.org/10.1016/j.watres.2019.115039},
volume = {166},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The metabolisms of anaerobic ammonium oxidation (anammox) bacteria related to ammonia oxidation with nitrite reduction and autotrophic carbon fixation have been extensively observed. However, little is known about the specific metabolic pathways associated with oxygen detoxification and organic carbon utilization. To this end, we obtained high abundance of anammox species (∼50%) in a lab-scale continuous stirred-tank reactor (CSTR) at room temperature without strict anaerobic condition. The draft genome of the dominant anammox bacteria affiliated to Ca. Brocadia sp. was recovered. Its metabolic pathways and genes expression were reconstructed and examined through metagenomic and metatranscriptomic analyses. Interestingly, the results suggested that this anammox lineage likely performs oxygen detoxification with genes encoding superoxide dismutase (SOD) and cytochrome c peroxidase (Ccp). Moreover, the Ccp-activated hydrogen peroxide (intermediate of oxygen detoxification) reduction might be energetically beneficial for the observed acetate conversion related to cell synthesis of Ca. Brocadia sp. This study offers a comprehensive understanding on the diverse metabolic activities in anammox species affiliated to Ca. Brocadia sp., and expanded the applicability of anammox process.
AU  - Ji,X
AU  - Wu,Z
AU  - Sung,S
AU  - Lee,P-H
DO  - 10.1016/j.watres.2019.115039
PY  - 2019///
TI  - Metagenomics and metatranscriptomics analyses reveal oxygen detoxification and mixotrophic potentials of an enriched anammox culture in a continuous stirred-tank reactor.
T2  - Water Res
UR  - http://dx.doi.org/10.1016/j.watres.2019.115039
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31520814
VL  - 166
ER  -
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