Imperial College London
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Dr Po-Heng (Henry) Lee

Faculty of Engineering, Department of Civil and Environmental Engineering

Senior Lecturer in Wastewater Engineering
 
 
 
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Contact

 

+44 (0)20 7594 5993po-heng.lee

 
 
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Location

 

310ASkempton BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Leng:2019:10.33430/V26N2THIE-2018-0041,
author = {Leng, L and Wang, Y and Yang, P and Narihiro, T and Nobu, MK and Tan, AGY and Lee, HPH},
doi = {10.33430/V26N2THIE-2018-0041},
journal = {HKIE Transactions Hong Kong Institution of Engineers},
pages = {63--71},
title = {Unravelling a microbial synergy to boost caproate production via carboxylates chain elongation with ethanol},
url = {http://dx.doi.org/10.33430/V26N2THIE-2018-0041},
volume = {26},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.
AU  - Leng,L
AU  - Wang,Y
AU  - Yang,P
AU  - Narihiro,T
AU  - Nobu,MK
AU  - Tan,AGY
AU  - Lee,HPH
DO  - 10.33430/V26N2THIE-2018-0041
EP  - 71
PY  - 2019///
SN  - 1023-697X
SP  - 63
TI  - Unravelling a microbial synergy to boost caproate production via carboxylates chain elongation with ethanol
T2  - HKIE Transactions Hong Kong Institution of Engineers
UR  - http://dx.doi.org/10.33430/V26N2THIE-2018-0041
VL  - 26
ER  -
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