Imperial College London
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Dr Po-Heng (Henry) Lee

Faculty of Engineering, Department of Civil and Environmental Engineering

Senior Lecturer in Wastewater Engineering
 
 
 
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Contact

 

+44 (0)20 7594 5993po-heng.lee

 
 
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Location

 

310ASkempton BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lam:2020:10.1016/j.watres.2020.115815,
author = {Lam, TYC and Mei, R and Wu, Z and Lee, PKH and Liu, W-T and Lee, P-H},
doi = {10.1016/j.watres.2020.115815},
journal = {Water Res},
title = {Superior resolution characterisation of microbial diversity in anaerobic digesters using full-length 16S rRNA gene amplicon sequencing.},
url = {http://dx.doi.org/10.1016/j.watres.2020.115815},
volume = {178},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In the past decade, the characterisation of the microbial community in anaerobic digestion was primarily done by using high-throughput short-read amplicon sequencing. However, the short-read approach has inherent primer bias and low phylogenetic resolution. Our previous study using Illumina MiSeq suggested that the heterogeneity of AD microbiome was operation-driven. To advance our knowledge towards the complexity of the AD microbiome, we performed full-length 16S rRNA gene amplicon sequencing using PacBio Sequel for a more accurate phylogenetic identification. To this end, purified DNA samples from 19 global anaerobic digesters were sequenced. Sixteen methanogenic archaea were identified at the species level. Among them, Methanosarcina horonobensis and Methanosarcina flavescens had significant presence under specific operating conditions. Methanothrix concilii presented in all digesters sequenced. Unexpectedly, over 90% of the Smithella detected were closely related to alkane-degrading Smithella strains D17 and M82, not Smithella propionica. Using LEfSe and network analysis, the interspecies relationship between the fermentative and syntrophic bacteria was addressed. Comparison of the short- and long-read sequencing results were performed and discussed. From sample preparation to data analysis, this work characterised the digester microbiomes in a superior resolution.
AU  - Lam,TYC
AU  - Mei,R
AU  - Wu,Z
AU  - Lee,PKH
AU  - Liu,W-T
AU  - Lee,P-H
DO  - 10.1016/j.watres.2020.115815
PY  - 2020///
TI  - Superior resolution characterisation of microbial diversity in anaerobic digesters using full-length 16S rRNA gene amplicon sequencing.
T2  - Water Res
UR  - http://dx.doi.org/10.1016/j.watres.2020.115815
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32380296
VL  - 178
ER  -
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