Imperial College London
Portrait Picture

Dr Po-Heng (Henry) Lee

Faculty of Engineering, Department of Civil and Environmental Engineering

Senior Lecturer in Wastewater Engineering
 
 
 
//

Contact

 

+44 (0)20 7594 5993po-heng.lee

 
 
//

Location

 

310ASkempton BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Lee:2020:10.3390/microorganisms8060919,
author = {Lee, P-H},
doi = {10.3390/microorganisms8060919},
journal = {Microorganisms},
pages = {1--13},
title = {Energy-efficient single-stage nitrite shunt denitrification with saline sewage through concise dissolved oxygen (DO) supply: Process performance and microbial communities},
url = {http://dx.doi.org/10.3390/microorganisms8060919},
volume = {8},
year = {2020}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Single-stage nitrite shunt denitrification (through nitrite rather than nitrate) with low dissolved oxygen (DO) supply is a better alternative in terms of energy-efficiency, short-footprint, and low C/N-ratio requirement. This study investigates the optimal DO level with temperature effect, with saline sewage at the fixed hydraulic and solids retention times of 8 h and 8 d, respectively. Moreover, 16S rRNA gene sequencing analysis corresponding with total nitrogen (TN) and chemical oxygen demand (COD) removals in each operating condition were performed. Results showed that DO of 0.3 mg/L at 20 °C achieved over 60.7% and over 97.9% of TN and COD removal, respectively, suggesting that such condition achieved effective nitrite-oxidizing bacteria inhibition and efficient denitrification. An unexpected finding was that sulfur-reducing Haematobacter and nitrogen-fixing Geofilum and Shinella were highly abundant with the copredominance of ammonia-oxidizing Comamonas and Nitrosomonas, nitrite-oxidizing Limnohabitans, and denitrifying Simplicispira, Castellaniella, and Nitratireductor. Further, canonical correspondence analysis (CCA) with respect to the operating conditions associated with phenotype prediction via R-based tool Tax4Fun was performed for a preliminary diagnosis of microbial functionality. The effects of DO, temperature, nitrite, and nitrate in various extents toward each predominant microbe were discussed. Collectively, DO is likely pivotal in single-stage nitrite shunt denitrification, as well as microbial communities, for energy-efficient saline sewage treatment.
AU  - Lee,P-H
DO  - 10.3390/microorganisms8060919
EP  - 13
PY  - 2020///
SN  - 2076-2607
SP  - 1
TI  - Energy-efficient single-stage nitrite shunt denitrification with saline sewage through concise dissolved oxygen (DO) supply: Process performance and microbial communities
T2  - Microorganisms
UR  - http://dx.doi.org/10.3390/microorganisms8060919
UR  - https://www.mdpi.com/2076-2607/8/6/919
UR  - http://hdl.handle.net/10044/1/81040
VL  - 8
ER  -
Download