Imperial College London

ProfessorRosemaryBoyton

Faculty of MedicineDepartment of Infectious Disease

Professor of Immunology and Respiratory Medicine
 
 
 
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Contact

 

r.boyton

 
 
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Location

 

8N22Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Boyton:2019:10.4049/jimmunol.1801453,
author = {Boyton, R and Reynolds, C and Chong, D and Li, Y and Black, L and Cutler, A and Webster, Z and Manji, J and Altmann, D},
doi = {10.4049/jimmunol.1801453},
journal = {Journal of Immunology},
pages = {2502--2510},
title = {Bioluminescent reporting of in vivo interferon gamma immune responses during infection and autoimmunity},
url = {http://dx.doi.org/10.4049/jimmunol.1801453},
volume = {202},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - IFN-γ is a key cytokine of innate and adaptive immunity. It is important to understand temporal changes in IFN-γ production and how these changes relate to the role of IFN-γ in diverse models of infectious and autoimmune disease, making the ability to monitor and track IFN-γ production in vivo of a substantial benefit. IFN-γ ELISPOTs have been a central methodology to measure T cell immunity for many years. In this study, we add the capacity to analyze IFN-γ responses with high sensitivity and specificity, longitudinally, in vitro and in vivo. This allows the refinement of experimental protocols because immunity can be tracked in real-time through a longitudinal approach. We have generated a novel murine IFN-γ reporter transgenic model that allows IFN-γ production to be visualized and quantified in vitro and in vivo as bioluminescence using an imaging system. At baseline, in the absence of an inflammatory stimulus, IFN-γ signal from lymphoid tissue is detectable in vivo. Reporter transgenics are used in this study to track the IFN-γ response to Pseudomonas aeruginosa infection in the lung over time in vivo. The longitudinal development of the adaptive T cell immunity following immunization with Ag is identified from day 7 in vivo. Finally, we show that we are able to use this reporter transgenic to follow the onset of autoimmune T cell activation after regulatory T cell depletion in an established model of systemic autoimmunity. This IFN-γ reporter transgenic, termed “Gammaglow,” offers a valuable new modality for tracking IFN-γ immunity, noninvasively and longitudinally over time.
AU - Boyton,R
AU - Reynolds,C
AU - Chong,D
AU - Li,Y
AU - Black,L
AU - Cutler,A
AU - Webster,Z
AU - Manji,J
AU - Altmann,D
DO - 10.4049/jimmunol.1801453
EP - 2510
PY - 2019///
SN - 1550-6606
SP - 2502
TI - Bioluminescent reporting of in vivo interferon gamma immune responses during infection and autoimmunity
T2 - Journal of Immunology
UR - http://dx.doi.org/10.4049/jimmunol.1801453
UR - https://www.jimmunol.org/content/202/8/2502
UR - http://hdl.handle.net/10044/1/73007
VL - 202
ER -