Imperial College London

ProfessorRosemaryBoyton

Faculty of MedicineDepartment of Infectious Disease

Professor of Immunology and Respiratory Medicine
 
 
 
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Contact

 

r.boyton

 
 
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Location

 

8N22Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Quigley:2015:10.1164/rccm.201411-1995OC,
author = {Quigley, KJ and Reynolds, CJ and Goudet, A and Raynsford, EJ and Sergeant, R and Quigley, A and Worgall, S and Bilton, D and Wilson, R and Loebinger, MR and Maillere, B and Altmann, DM and Boyton, RJ},
doi = {10.1164/rccm.201411-1995OC},
journal = {American Journal of Respiratory and Critical Care Medicine},
title = {Chronic infection by Mucoid Pseudomonas aeruginosa associated with dysregulation in T cell immunity to OprF.},
url = {http://dx.doi.org/10.1164/rccm.201411-1995OC},
volume = {191},
year = {2015}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - RATIONALE: Pseudomonas aeruginosa (PA) is an environmental pathogen that commonly infects individuals with cystic fibrosis (CF) and non-CF bronchiectasis, impacting on morbidity and mortality. To understand the pathobiology of interactions between the bacterium and host adaptive immunity and to inform rational vaccine design, it is important to understand the adaptive immune correlates of disease. OBJECTIVES: We characterized T cell immunity to the PA antigen, outer membrane porin F (OprF), analyzing immunodominant epitopes in relation to infection status. METHODS: Non-CF bronchiectasis patients were stratified by frequency of PA isolation. T cell IFNγ immunity to OprF and its immunodominant epitopes was characterized. Patterns of HLA-restriction of immunodominant epitopes were defined using HLA class II transgenic mice. Immunity was characterized with respect to cytokine and chemokine secretion, antibody response and T cell activation transcripts. MEASUREMENTS AND MAIN RESULTS: Patients were stratified according to whether PA was never, sometimes (<50%) or frequently (>or=50%) isolated from sputum. Patients with frequent PA sputum-positive isolates were more likely to be infected by mucoid PA, and showed a narrow T cell epitope response and a relative reduction in Th1 polarizing transcription factors, but enhanced immunity with respect to antibody production, innate cytokines and chemokines. CONCLUSIONS: We have defined the immunodominant, HLA-restricted, T cell epitopes of OprF. Our observation that chronic infection is associated with a response of narrowed specificity, despite strong innate and antibody immunity, may help to explain susceptibility in these individuals and pave the way for better vaccine design to achieve protective immunity.
AU - Quigley,KJ
AU - Reynolds,CJ
AU - Goudet,A
AU - Raynsford,EJ
AU - Sergeant,R
AU - Quigley,A
AU - Worgall,S
AU - Bilton,D
AU - Wilson,R
AU - Loebinger,MR
AU - Maillere,B
AU - Altmann,DM
AU - Boyton,RJ
DO - 10.1164/rccm.201411-1995OC
PY - 2015///
SN - 1073-449X
TI - Chronic infection by Mucoid Pseudomonas aeruginosa associated with dysregulation in T cell immunity to OprF.
T2 - American Journal of Respiratory and Critical Care Medicine
UR - http://dx.doi.org/10.1164/rccm.201411-1995OC
UR - http://hdl.handle.net/10044/1/23374
VL - 191
ER -