Imperial College London
Alternate

ProfessorRosemaryBoyton

Faculty of Medicine, Department of Infectious Disease

Professor of Immunology and Respiratory Medicine
 
 
 
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Contact

 

r.boyton

 
 
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Location

 

8N22Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Manisty:2021:10.1016/j.ebiom.2021.103259,
author = {Manisty, C and Treibel, TA and Jensen, M and Semper, A and Joy, G and Gupta, RK and Cutino-Moguel, T and Andiapen, M and Jones, J and Taylor, S and Otter, A and Pade, C and Gibbons, J and Lee, J and Bacon, J and Thomas, S and Moon, C and Jones, M and Williams, D and Lambourne, J and Fontana, M and Altmann, DM and Boyton, R and Maini, M and McKnight, A and Chain, B and Noursadeghi, M and Moon, JC},
doi = {10.1016/j.ebiom.2021.103259},
journal = {EBioMedicine},
title = {Time series analysis and mechanistic modelling of heterogeneity and sero-reversion in antibody responses to mild SARSCoV-2 infection},
url = {http://dx.doi.org/10.1016/j.ebiom.2021.103259},
volume = {65},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: SARS-CoV-2 serology is used to identify prior infection at individual and at population level. Extended longitudinal studies with multi-timepoint sampling to evaluate dynamic changes in antibody levels are required to identify the time horizon in which these applications of serology are valid, and to explore the longevity of protective humoral immunity. METHODS: Healthcare workers were recruited to a prospective cohort study from the first SARS-CoV-2 epidemic peak in London, undergoing weekly symptom screen, viral PCR and blood sampling over 16-21 weeks. Serological analysis (n =12,990) was performed using semi-quantitative Euroimmun IgG to viral spike S1 domain and Roche total antibody to viral nucleocapsid protein (NP) assays. Comparisons were made to pseudovirus neutralizing antibody measurements. FINDINGS: A total of 157/729 (21.5%) participants developed positive SARS-CoV-2 serology by one or other assay, of whom 31.0% were asymptomatic and there were no deaths. Peak Euroimmun anti-S1 and Roche anti-NP measurements correlated (r = 0.57, p<0.0001) but only anti-S1 measurements correlated with near-contemporary pseudovirus neutralising antibody titres (measured at 16-18 weeks, r = 0.57, p<0.0001). By 21 weeks' follow-up, 31/143 (21.7%) anti-S1 and 6/150 (4.0%) anti-NP measurements reverted to negative. Mathematical modelling revealed faster clearance of anti-S1 compared to anti-NP (median half-life of 2.5 weeks versus 4.0 weeks), earlier transition to lower levels of antibody production (median of 8 versus 13 weeks), and greater reductions in relative antibody production rate after the transition (median of 35% versus 50%). INTERPRETATION: Mild SARS-CoV-2 infection is associated with heterogeneous serological responses in Euroimmun anti-S1 and Roche anti-NP assays. Anti-S1 responses showed faster rates of clearance, more rapid transition from high to low level production rate and greater reduction in production rate
AU  - Manisty,C
AU  - Treibel,TA
AU  - Jensen,M
AU  - Semper,A
AU  - Joy,G
AU  - Gupta,RK
AU  - Cutino-Moguel,T
AU  - Andiapen,M
AU  - Jones,J
AU  - Taylor,S
AU  - Otter,A
AU  - Pade,C
AU  - Gibbons,J
AU  - Lee,J
AU  - Bacon,J
AU  - Thomas,S
AU  - Moon,C
AU  - Jones,M
AU  - Williams,D
AU  - Lambourne,J
AU  - Fontana,M
AU  - Altmann,DM
AU  - Boyton,R
AU  - Maini,M
AU  - McKnight,A
AU  - Chain,B
AU  - Noursadeghi,M
AU  - Moon,JC
DO  - 10.1016/j.ebiom.2021.103259
PY  - 2021///
SN  - 2352-3964
TI  - Time series analysis and mechanistic modelling of heterogeneity and sero-reversion in antibody responses to mild SARSCoV-2 infection
T2  - EBioMedicine
UR  - http://dx.doi.org/10.1016/j.ebiom.2021.103259
UR  - https://www.ncbi.nlm.nih.gov/pubmed/33662833
UR  - http://hdl.handle.net/10044/1/86399
VL  - 65
ER  -
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