Imperial College London
Portrait Picture

DrRashedaChowdhury

Faculty of Medicine, National Heart & Lung Institute

Advanced Research Fellow
 
 
 
//

Contact

 

r.chowdhury

 
 
//

Location

 

ICTEM buildingHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Kanemaru:2023:10.1038/s41586-023-06311-1,
author = {Kanemaru, K and Cranley, J and Muraro, D and Miranda, AMA and Ho, SY and Wilbrey-Clark, A and Patrick, Pett J and Polanski, K and Richardson, L and Litvinukova, M and Kumasaka, N and Qin, Y and Jablonska, Z and Semprich, CI and Mach, L and Dabrowska, M and Richoz, N and Bolt, L and Mamanova, L and Kapuge, R and Barnett, SN and Perera, S and Talavera-López, C and Mulas, I and Mahbubani, KT and Tuck, L and Wang, L and Huang, MM and Prete, M and Pritchard, S and Dark, J and Saeb-Parsy, K and Patel, M and Clatworthy, MR and Hübner, N and Chowdhury, RA and Noseda, M and Teichmann, SA},
doi = {10.1038/s41586-023-06311-1},
journal = {Nature},
pages = {801--810},
title = {Spatially resolved multiomics of human cardiac niches},
url = {http://dx.doi.org/10.1038/s41586-023-06311-1},
volume = {619},
year = {2023}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The function of a cell is defined by its intrinsic characteristics and its niche: the tissue microenvironment in which it dwells. Here we combine single-cell and spatial transcriptomics data to discover cellular niches within eight regions of the human heart. We map cells to microanatomical locations and integrate knowledge-based and unsupervised structural annotations. We also profile the cells of the human cardiac conduction system1. The results revealed their distinctive repertoire of ion channels, G-protein-coupled receptors (GPCRs) and regulatory networks, and implicated FOXP2 in the pacemaker phenotype. We show that the sinoatrial node is compartmentalized, with a core of pacemaker cells, fibroblasts and glial cells supporting glutamatergic signalling. Using a custom CellPhoneDB.org module, we identify trans-synaptic pacemaker cell interactions with glia. We introduce a druggable target prediction tool, drug2cell, which leverages single-cell profiles and drug-target interactions to provide mechanistic insights into the chronotropic effects of drugs, including GLP-1 analogues. In the epicardium, we show enrichment of both IgG+ and IgA+ plasma cells forming immune niches that may contribute to infection defence. Overall, we provide new clarity to cardiac electro-anatomy and immunology, and our suite of computational approaches can be applied to other tissues and organs.
AU  - Kanemaru,K
AU  - Cranley,J
AU  - Muraro,D
AU  - Miranda,AMA
AU  - Ho,SY
AU  - Wilbrey-Clark,A
AU  - Patrick,Pett J
AU  - Polanski,K
AU  - Richardson,L
AU  - Litvinukova,M
AU  - Kumasaka,N
AU  - Qin,Y
AU  - Jablonska,Z
AU  - Semprich,CI
AU  - Mach,L
AU  - Dabrowska,M
AU  - Richoz,N
AU  - Bolt,L
AU  - Mamanova,L
AU  - Kapuge,R
AU  - Barnett,SN
AU  - Perera,S
AU  - Talavera-López,C
AU  - Mulas,I
AU  - Mahbubani,KT
AU  - Tuck,L
AU  - Wang,L
AU  - Huang,MM
AU  - Prete,M
AU  - Pritchard,S
AU  - Dark,J
AU  - Saeb-Parsy,K
AU  - Patel,M
AU  - Clatworthy,MR
AU  - Hübner,N
AU  - Chowdhury,RA
AU  - Noseda,M
AU  - Teichmann,SA
DO  - 10.1038/s41586-023-06311-1
EP  - 810
PY  - 2023///
SN  - 0028-0836
SP  - 801
TI  - Spatially resolved multiomics of human cardiac niches
T2  - Nature
UR  - http://dx.doi.org/10.1038/s41586-023-06311-1
UR  - https://www.ncbi.nlm.nih.gov/pubmed/37438528
UR  - https://www.nature.com/articles/s41586-023-06311-1
UR  - http://hdl.handle.net/10044/1/105964
VL  - 619
ER  -
Download