15 results found
Gray REJ, Ewers RM, Boyle MJW, et al., 2018, Effect of tropical forest disturbance on the competitive interactions within a diverse ant community, SCIENTIFIC REPORTS, Vol: 8, ISSN: 2045-2322
Samuelson AE, Gill RJ, Brown MJF, et al., 2018, Lower bumblebee colony reproductive success in agricultural compared with urban environments, PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 285, ISSN: 0962-8452
Arce AN, David TI, Randall EL, et al., 2017, Impact of controlled neonicotinoid exposure on bumblebees in a realistic field setting, JOURNAL OF APPLIED ECOLOGY, Vol: 54, Pages: 1199-1208, ISSN: 0021-8901
Bohan DA, Landuyt D, Ma A, et al., 2016, Networking Our Way to Better Ecosystem Service Provision, TRENDS IN ECOLOGY & EVOLUTION, Vol: 31, Pages: 105-115, ISSN: 0169-5347
Gill RJ, Baldock KCR, Brown MJF, et al., 2016, Protecting an Ecosystem Service: Approaches to Understanding and Mitigating Threats to Wild Insect Pollinators, ECOSYSTEM SERVICES: FROM BIODIVERSITY TO SOCIETY, PT 2, Vol: 54, Pages: 135-206, ISSN: 0065-2504
Samuelson EEW, Chen-Wishart ZP, Gill RJ, et al., 2016, Effect of acute pesticide exposure on bee spatial working memory using an analogue of the radial-arm maze, SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322
Smith DB, Bernhardt G, Raine NE, et al., 2016, Exploring miniature insect brains using micro-CT scanning techniques., Sci Rep, Vol: 6
The capacity to explore soft tissue structures in detail is important in understanding animal physiology and how this determines features such as movement, behaviour and the impact of trauma on regular function. Here we use advances in micro-computed tomography (micro-CT) technology to explore the brain of an important insect pollinator and model organism, the bumblebee (Bombus terrestris). Here we present a method for accurate imaging and exploration of insect brains that keeps brain tissue free from trauma and in its natural stereo-geometry, and showcase our 3D reconstructions and analyses of 19 individual brains at high resolution. Development of this protocol allows relatively rapid and cost effective brain reconstructions, making it an accessible methodology to the wider scientific community. The protocol describes the necessary steps for sample preparation, tissue staining, micro-CT scanning and 3D reconstruction, followed by a method for image analysis using the freeware SPIERS. These image analysis methods describe how to virtually extract key composite structures from the insect brain, and we demonstrate the application and precision of this method by calculating structural volumes and investigating the allometric relationships between bumblebee brain structures.
Raine NE, Gill RJ, 2015, Ecology: Tasteless pesticides affect bees in the field., Nature, Vol: 521, Pages: 38-40
Gill RJ, Raine NE, 2014, Chronic impairment of bumblebee natural foraging behaviour induced by sublethal pesticide exposure, FUNCTIONAL ECOLOGY, Vol: 28, Pages: 1459-1471, ISSN: 0269-8463
Bryden J, Gill RJ, Mitton RAA, et al., 2013, Chronic sublethal stress causes bee colony failure, ECOLOGY LETTERS, Vol: 16, Pages: 1463-1469, ISSN: 1461-023X
Gill RJ, Ramos-Rodriguez O, Raine NE, 2012, Combined pesticide exposure severely affects individual- and colony-level traits in bees., Nature, Vol: 491, Pages: 105-108
Reported widespread declines of wild and managed insect pollinators have serious consequences for global ecosystem services and agricultural production. Bees contribute approximately 80% of insect pollination, so it is important to understand and mitigate the causes of current declines in bee populations . Recent studies have implicated the role of pesticides in these declines, as exposure to these chemicals has been associated with changes in bee behaviour and reductions in colony queen production. However, the key link between changes in individual behaviour and the consequent impact at the colony level has not been shown. Social bee colonies depend on the collective performance of many individual workers. Thus, although field-level pesticide concentrations can have subtle or sublethal effects at the individual level, it is not known whether bee societies can buffer such effects or whether it results in a severe cumulative effect at the colony level. Furthermore, widespread agricultural intensification means that bees are exposed to numerous pesticides when foraging, yet the possible combinatorial effects of pesticide exposure have rarely been investigated. Here we show that chronic exposure of bumblebees to two pesticides (neonicotinoid and pyrethroid) at concentrations that could approximate field-level exposure impairs natural foraging behaviour and increases worker mortality leading to significant reductions in brood development and colony success. We found that worker foraging performance, particularly pollen collecting efficiency, was significantly reduced with observed knock-on effects for forager recruitment, worker losses and overall worker productivity. Moreover, we provide evidence that combinatorial exposure to pesticides increases the propensity of colonies to fail.
Coston DJ, Gill RJ, Hammond RL, 2011, No evidence of volatile chemicals regulating reproduction in a multiple queen ant, NATURWISSENSCHAFTEN, Vol: 98, Pages: 625-629, ISSN: 0028-1042
Gill RJ, Hammond RL, 2011, Workers influence royal reproduction, PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 278, Pages: 1524-1531, ISSN: 0962-8452
Gill RJ, Hammond RL, 2011, Workers determine queen inheritance of reproduction in a functionally monogynous ant population, ANIMAL BEHAVIOUR, Vol: 82, Pages: 119-129, ISSN: 0003-3472
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