Imperial College London
Professor Robert Wilkinson

ProfessorRobertWilkinson

Faculty of Medicine, Department of Infectious Disease

Professor in Infectious Diseases
 
 
 
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Contact

 

r.j.wilkinson Website

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Riou:2022:10.1183/13993003.00285-2021,
author = {Riou, C and Schafer, G and du, Bruyn E and Goliath, RT and Stek, C and Mou, H and Hung, D and Wilkinson, KA and Wilkinson, R},
doi = {10.1183/13993003.00285-2021},
journal = {European Respiratory Journal},
title = {Rapid, simplified whole blood-based multiparameter assay to quantify and phenotype SARS-CoV-2 specific T cells},
url = {http://dx.doi.org/10.1183/13993003.00285-2021},
volume = {59},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Rapid tests to evaluate SARS-CoV-2-specific T cell responses are urgently needed to decipher protective immunity and aid monitoring vaccine-induced immunity. Using a rapid whole blood assay requiring minimal amount of blood, we measured qualitatively and quantitatively SARS-38CoV-2-specific CD4 T cell responses in 31 healthcare workers, using flow cytometry. 100% of COVID-19 convalescent participants displayed a detectable SARS-CoV-2-specific CD4 T cell response. SARS-CoV-2-responding cells were also detected in 40.9% of participants with no COVID-19-associated symptoms or who tested PCR negative. Phenotypic assessment indicated that, in COVID-19 convalescent participants, SARS-CoV-2 CD4 responses displayed an early differentiated memory phenotype with limited capacity to produce IFN. Conversely, in participants with no reported symptoms, SARS-CoV-2 CD4 responses were enriched in late differentiated cells, co-expressing IFN and TNFα and also Granzyme B. This proof-of-concept study presents a scalable alternative to PBMC-based assays to enumerate and phenotype SARS-CoV-2-responding T cells, thus representing a practical tool to monitor adaptive immunity due to natural infection or vaccine trials.
AU  - Riou,C
AU  - Schafer,G
AU  - du,Bruyn E
AU  - Goliath,RT
AU  - Stek,C
AU  - Mou,H
AU  - Hung,D
AU  - Wilkinson,KA
AU  - Wilkinson,R
DO  - 10.1183/13993003.00285-2021
PY  - 2022///
SN  - 0903-1936
TI  - Rapid, simplified whole blood-based multiparameter assay to quantify and phenotype SARS-CoV-2 specific T cells
T2  - European Respiratory Journal
UR  - http://dx.doi.org/10.1183/13993003.00285-2021
UR  - http://hdl.handle.net/10044/1/89090
VL  - 59
ER  -
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