Imperial College London
Professor Robert Wilkinson

ProfessorRobertWilkinson

Faculty of Medicine, Department of Infectious Disease

Professor in Infectious Diseases
 
 
 
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Contact

 

r.j.wilkinson Website

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bunjun:2021:10.3389/fimmu.2021.707355,
author = {Bunjun, R and Soares, AP and Thawer, N and Müller, TL and Kiravu, A and Ginbot, Z and Corleis, B and Murugan, BD and Kwon, DS and Groote-Bidlingmaier, FV and Riou, C and Wilkinson, RJ and Walzl, G and Burgers, W},
doi = {10.3389/fimmu.2021.707355},
journal = {Frontiers in Immunology},
pages = {1--12},
title = {Dysregulation of the immune environment in the airways during HIV infection},
url = {http://dx.doi.org/10.3389/fimmu.2021.707355},
volume = {12},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - HIV-1 increases susceptibility to pulmonary infection and disease, suggesting pathogenesis in the lung. However, the lung immune environment during HIV infection remains poorly characterized. This study examined T cell activation and the cytokine milieu in paired bronchoalveolar lavage (BAL) and blood from 36 HIV-uninfected and 32 HIV-infected participants. Concentrations of 27 cytokines were measured by Luminex, and T cells were phenotyped by flow cytometry. Blood and BAL had distinct cytokine profiles (p=0.001). In plasma, concentrations of inflammatory cytokines like IFN-γ (p=0.004) and TNF-α (p=0.004) were elevated during HIV infection, as expected. Conversely, BAL cytokine concentrations were similar in HIV-infected and uninfected individuals, despite high BAL viral loads (VL; median 48,000 copies/ml epithelial lining fluid). HIV-infected individuals had greater numbers of T cells in BAL compared to uninfected individuals (p=0.007); and BAL VL positively associated with CD4+ and CD8+ T cell numbers (p=0.006 and p=0.0002, respectively) and CXCL10 concentrations (p=0.02). BAL T cells were highly activated in HIV-infected individuals, with nearly 2-3 fold greater frequencies of CD4+CD38+ (1.8-fold; p=0.007), CD4+CD38+HLA-DR+ (1.9-fold; p=0.0006), CD8+CD38+ (2.8-fold; p=0.0006), CD8+HLA-DR+ (2-fold; p=0.022) and CD8+CD38+HLA-DR+ (3.6-fold; p<0.0001) cells compared to HIV-uninfected individuals. Overall, this study demonstrates a clear disruption of the pulmonary immune environment during HIV infection, with readily detectable virus and activated T lymphocytes, which may be driven to accumulate by local chemokines.
AU  - Bunjun,R
AU  - Soares,AP
AU  - Thawer,N
AU  - Müller,TL
AU  - Kiravu,A
AU  - Ginbot,Z
AU  - Corleis,B
AU  - Murugan,BD
AU  - Kwon,DS
AU  - Groote-Bidlingmaier,FV
AU  - Riou,C
AU  - Wilkinson,RJ
AU  - Walzl,G
AU  - Burgers,W
DO  - 10.3389/fimmu.2021.707355
EP  - 12
PY  - 2021///
SN  - 1664-3224
SP  - 1
TI  - Dysregulation of the immune environment in the airways during HIV infection
T2  - Frontiers in Immunology
UR  - http://dx.doi.org/10.3389/fimmu.2021.707355
UR  - https://www.frontiersin.org/articles/10.3389/fimmu.2021.707355/full
UR  - http://hdl.handle.net/10044/1/90245
VL  - 12
ER  -
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