Imperial College London
Alternate

Professor Kitney

Faculty of Engineering, Department of Bioengineering

Professor of BioMedical Systems Engineering
 
 
 
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Contact

 

+44 (0)20 7594 6226r.kitney Website

 
 
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Assistant

 

Ms Tania Briggs +44 (0)20 7594 6226

 
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Location

 

3.16Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wang:2011:10.1038/ncomms1516,
author = {Wang, B and Kitney, RI and Joly, N and Buck, M},
doi = {10.1038/ncomms1516},
journal = {Nature Communications},
title = {Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology},
url = {http://dx.doi.org/10.1038/ncomms1516},
volume = {2:508},
year = {2011}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Modular and orthogonal genetic logic gates are essential for building robust biologically based digital devices to customize cell signalling in synthetic biology. Here we constructed an orthogonal AND gate in Escherichia coli using a novel hetero-regulation module from Pseudomonas syringae. The device comprises two co-activating genes hrpR and hrpS controlled by separate promoter inputs, and a σ54-dependent hrpL promoter driving the output. The hrpL promoter is activated only when both genes are expressed, generating digital-like AND integration behaviour. The AND gate is demonstrated to be modular by applying new regulated promoters to the inputs, and connecting the output to a NOT gate module to produce a combinatorial NAND gate. The circuits were assembled using a parts-based engineering approach of quantitative characterization, modelling, followed by construction and testing. The results show that new genetic logic devices can be engineered predictably from novel native orthogonal biological control elements using quantitatively in-context characterized parts.
AU  - Wang,B
AU  - Kitney,RI
AU  - Joly,N
AU  - Buck,M
DO  - 10.1038/ncomms1516
PY  - 2011///
TI  - Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms1516
UR  - http://hdl.handle.net/10044/1/15510
VL  - 2:508
ER  -
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