Imperial College London

ProfessorRobKrams

Faculty of EngineeringDepartment of Bioengineering

Professor of Molecular Bioengineering
 
 
 
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Contact

 

+44 (0)20 7594 1473r.krams

 
 
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Location

 

3.15Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

290 results found

Denis J-F, Scheckenbach KEL, Pfenniger A, Meens MJ, Krams R, Miquerol L, Taffet S, Chanson M, Delmar M, Kwak BRet al., 2017, Connexin40 controls endothelial activation by dampening NF kappa B activation, ONCOTARGET, Vol: 8, Pages: 50972-50986, ISSN: 1949-2553

JOURNAL ARTICLE

Mohri Z, Hernandez ADR, Krams R, 2017, The emerging role of YAP/TAZ in mechanotransduction, JOURNAL OF THORACIC DISEASE, Vol: 9, Pages: E507-E509, ISSN: 2072-1439

JOURNAL ARTICLE

Pedrigi RM, Papadimitriou KI, Kondiboyina A, Sidhu S, Chau J, Patel MB, Baeriswyl DC, Drakakis EM, Krams Ret al., 2017, Disturbed Cyclical Stretch of Endothelial Cells Promotes Nuclear Expression of the Pro-Atherogenic Transcription Factor NF-kappa B, ANNALS OF BIOMEDICAL ENGINEERING, Vol: 45, Pages: 898-909, ISSN: 0090-6964

JOURNAL ARTICLE

Poulsen CB, Pedrigi R, Holm N, Pareek N, Kilic I, Post AL, Di Mario C, Bentzon J, Botker H, Falk E, Krams R, de Silva Ret al., 2017, COMPARISON OF IN VIVO OPTICAL COHERENCE TOMOGRAPHY DERIVED PLAQUE PHENOTYPE AND BURDEN WITH HISTOLOGY IN A PORCINE MODEL OF ADVANCED CORONARY ATHEROSCLEROSIS, 66th Annual Scientific Session and Expo of the American-College-of-Cardiology (ACC), Publisher: ELSEVIER SCIENCE INC, Pages: 1072-1072, ISSN: 0735-1097

CONFERENCE PAPER

Seneviratne AN, Edsfeldt A, Cole JE, Kassiteridi C, Swart M, Park I, Green P, Khoyratty T, Saliba D, Goddard ME, Sansom SN, Goncalves I, Krams R, Udalova IA, Monaco Cet al., 2017, Interferon Regulatory Factor 5 Controls Necrotic Core Formation in Atherosclerotic Lesions by Impairing Efferocytosis, CIRCULATION, Vol: 136, Pages: 1140-+, ISSN: 0009-7322

JOURNAL ARTICLE

Serbanovic-Canic J, de Luca A, Warboys C, Ferreira PF, Luong LA, Hsiao S, Gauci I, Mahmoud M, Feng S, Souilhol C, Bowden N, Ashton J-P, Walczak H, Firmin D, Krams R, Mason JC, Haskard DO, Sherwin S, Ridger V, Chico TJA, Evans PCet al., 2017, Zebrafish Model for Functional Screening of Flow-Responsive Genes, ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, Vol: 37, Pages: 130-+, ISSN: 1079-5642

JOURNAL ARTICLE

Antonacci G, Pedrigi R, Krams R, Torok Pet al., 2016, Quantification of plaque stiffness by Brillouin microscopy (Conference Presentation), 3rd Conference on Optical Elastography and Tissue Biomechanics III, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

CONFERENCE PAPER

Kis Z, Rodin T, Zafar A, Lai Z, Freke G, Fleck O, Hernandez ADR, Towhidi L, Pedrigi RM, Homma T, Krams Ret al., 2016, Development of a synthetic gene network to modulate gene expression by mechanical forces, SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322

JOURNAL ARTICLE

Krams RM, Pedrigi RM, Kis Z, 2016, A novel assay for regulating transcription factors by flow, CARDIOVASCULAR RESEARCH, Vol: 111, Pages: S117-S117, ISSN: 0008-6363

JOURNAL ARTICLE

Maimari N, Pedrigi RM, Russo A, Broda K, Krams Ret al., 2016, Integration of flow studies for robust selection of mechanoresponsive genes, THROMBOSIS AND HAEMOSTASIS, Vol: 115, Pages: 474-483, ISSN: 0340-6245

JOURNAL ARTICLE

Pedrigi RM, Mehta VV, Bovens SM, Mohri Z, Poulsen CB, Gsell W, Tremoleda JL, Towhidi L, de Silva R, Petretto E, Krams Ret al., 2016, Influence of shear stress magnitude and direction on atherosclerotic plaque composition, Royal Society Open Science, Vol: 3, ISSN: 2054-5703

JOURNAL ARTICLE

Seneviratne AN, Cole JE, Goddard ME, Park I, Mohri Z, Sansom S, Udalova I, Krams R, Monaco Cet al., 2016, "Low shear stress induces M1 macrophage polarization in murine thin-cap atherosclerotic plaques" (vol 89B, pg 168, 2015), JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 91, Pages: 10-10, ISSN: 0022-2828

JOURNAL ARTICLE

Towhidi L, Khodadadi D, Maimari N, Pedrigi RM, Ip H, Kis Z, Kwak BR, Petrova TW, Delorenzi M, Krams Ret al., 2016, Comparison between direct and reverse electroporation of cells in situ: a simulation study, PHYSIOLOGICAL REPORTS, Vol: 4, ISSN: 2051-817X

JOURNAL ARTICLE

Antonacci G, Pedrigi RM, Kondiboyina A, Mehta VV, de Silva R, Paterson C, Krams R, Toeroek Pet al., 2015, Quantification of plaque stiffness by Brillouin microscopy in experimental thin cap fibroatheroma, JOURNAL OF THE ROYAL SOCIETY INTERFACE, Vol: 12, ISSN: 1742-5689

JOURNAL ARTICLE

Dent MR, Lopez-Duarte I, Dickson CJ, Geoghegan ND, Cooper JM, Gould IR, Krams R, Bull JA, Brooks NJ, Kuimova MKet al., 2015, Imaging phase separation in model lipid membranes through the use of BODIPY based molecular rotors, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 17, Pages: 18393-18402, ISSN: 1463-9076

JOURNAL ARTICLE

Kis Z, Pereira HS, Homma T, Pedrigi RM, Krams Ret al., 2015, Mammalian synthetic biology: emerging medical applications, JOURNAL OF THE ROYAL SOCIETY INTERFACE, Vol: 12, ISSN: 1742-5689

JOURNAL ARTICLE

Pedrigi RM, Poulsen CB, Mehta VV, Holm NR, Pareek N, Post AL, Kilic ID, Banya WAS, Dall'Ara G, Mattesini A, Bjorklund MM, Andersen NP, Grondal AK, Petretto E, Foin N, Davies JE, Di Mario C, Bentzon JF, Botker HE, Falk E, Krams R, de Silva Ret al., 2015, Inducing Persistent Flow Disturbances Accelerates Atherogenesis and Promotes Thin Cap Fibroatheroma Development in D374Y-PCSK9 Hypercholesterolemic Minipigs, CIRCULATION, Vol: 132, Pages: 1003-1012, ISSN: 0009-7322

JOURNAL ARTICLE

Pereira GDC, Krams R, 2015, FLOW CHARACTERISTICS ENHANCED BY THE COMPLIANT ARTERIES AND THEIR EFFECTS IN VULNERABLE PLAQUE FORMATION, British-Cardiac-Society (BCS) Annual Conference on Hearts and Genes, Publisher: BMJ PUBLISHING GROUP, ISSN: 1355-6037

CONFERENCE PAPER

Pfenniger A, Meens MJ, Pedrigi RM, Foglia B, Sutter E, Pelli G, Rochemont V, Petrova TV, Krams R, Kwak BRet al., 2015, Shear stress-induced atherosclerotic plaque composition in ApoE(-/-) mice is modulated by connexin37, ATHEROSCLEROSIS, Vol: 243, Pages: 1-10, ISSN: 0021-9150

JOURNAL ARTICLE

Poulsen CB, Pedrigi RM, Mehta VV, Post A, Pareek N, Holm NR, Kilic D, El-Bahnasawi M, Bjorklund MM, Andersen NP, Grondal AK, Nachiappan A, Foin N, Davies JER, Di Mario C, Botker HE, Bentzon JF, Falk E, Krams R, de Silva Ret al., 2015, INDUCTION OF PERTURBED SHEAR STRESS LEADS TO FOCAL ADVANCED ATHEROSCLEROTIC PLAQUE FORMATION IN TRANSGENIC MINIPIGS WITH HYPERCHOLESTEROLEMIA, Scientific Session of the American-College-of-Cardiology (ACC), Publisher: ELSEVIER SCIENCE INC, Pages: A1927-A1927, ISSN: 0735-1097

CONFERENCE PAPER

Shamout FE, Pouliopoulos AN, Lee P, Bonaccorsi S, Towhidi L, Krams R, Choi JJet al., 2015, ENHANCEMENT OF NON-INVASIVE TRANS-MEMBRANE DRUG DELIVERY USING ULTRASOUND AND MICROBUBBLES DURING PHYSIOLOGICALLY RELEVANT FLOW, ULTRASOUND IN MEDICINE AND BIOLOGY, Vol: 41, Pages: 2435-2448, ISSN: 0301-5629

JOURNAL ARTICLE

Villanueva ME, Pereira HSA, Rajyaguru J, Krams R, Chachuat Bet al., 2015, Bifurcation and stability analysis of nonlinear dynamic systems using complete search, Pages: 627-628

CONFERENCE PAPER

Caro CG, Seneviratne A, Heraty KB, Monaco C, Burke MG, Krams R, Chang CC, Gilson P, Coppola Get al., 2014, Intimal hyperplasia following implantation of helical-centreline and straight-centreline stents in common carotid arteries in healthy pigs: influence of intraluminal flow (vol 10, 20130578, 2013), JOURNAL OF THE ROYAL SOCIETY INTERFACE, Vol: 11, ISSN: 1742-5689

JOURNAL ARTICLE

Cuhlmann S, Gsell W, Van der Heiden K, Habib J, Tremoleda JL, Khalil M, Turkheimer F, Meens MJ, Kwak BR, Bird J, Davenport AP, Clark J, Haskard D, Krams R, Jones H, Evans PCet al., 2014, In Vivo Mapping of Vascular Inflammation Using the Translocator Protein Tracer F-18-FEDAA1106, MOLECULAR IMAGING, Vol: 13, ISSN: 1535-3508

JOURNAL ARTICLE

Cuhlmann S, Gsell W, Van der Heiden K, Habib J, Tremoleda JL, Khalil M, Turkheimer F, Meens MJ, Kwak BR, Bird J, Davenport AP, Clark J, Haskard D, Krams R, Jones H, Evans PCet al., 2014, In Vivo Mapping of Vascular Inflammation Using the Translocator Protein Tracer (18)F-FEDAA1106., Mol Imaging, Vol: 13

Noninvasive imaging methods are required to monitor the inflammatory content of atherosclerotic plaques. FEDAA1106 (N-(5-fluoro- 2-phenoxyphenyl)- N-(2-(2-fluoroethoxy)-5-methoxybenzyl) acetamide) is a selective ligand for TSPO-18kDa (also known as peripheral benzodiazepine receptor), which is expressed by activated macrophages. We compared (18)F-FEDAA1106 and 2-deoxy-2-[(18)F]fluoro-D- glucose ((18)F-FDG, a marker of glucose metabolism) for positron emission tomographic (PET) imaging of vascular inflammation. This was tested using a murine model in which focal inflammation was induced in the carotid artery via placement of a constrictive cuff. Immunostaining revealed CD68-positive cells (macrophages) at a disturbed flow site located downstream from the cuff. Dynamic PET imaging using (18)F-FEDAA1106 or (18)F-FDG was registered to anatomic data generated by computed tomographic (CT)/CT angiography. Standardized uptake values were significantly increased at cuffed compared to contralateral arteries using either (18)F-FEDAA1106 ( p < .01) or FDG ( p < .05). However, the (18)F-FEDAA1106 signal was significantly higher at the inflamed disturbed flow region compared to the noninflamed uniform flow regions, whereas differences in FDG uptake were less distinct. We conclude that (18)F-FEDAA1106 can be used in vivo for detection of vascular inflammation. Moreover, the signal pattern of (18)F-FEDAA1106 corresponded with vascular inflammation more specifically than FDG uptake.

JOURNAL ARTICLE

Homma T, Caurette P, Silva HJ, Yamada I, Makrydaki E, Bovens S, Pedrigi R, Petretto EG, Krams Ret al., 2014, TRANSCRIPTOME ANALYSIS OF LOCAL SHEAR DEPENDENT GENE EXPRESSION IN ENDOTHELIAL CELLS DURING THIN-CAP FIBROATHEROMA(TCFA) DEVELOPMENT, Autumn Meeting of the British-Atherosclerosis-Society (BAS), Publisher: ELSEVIER IRELAND LTD, Pages: E9-E9, ISSN: 0021-9150

CONFERENCE PAPER

Kis Z, Towhidi L, Ip H, Drakakis E, Bharath A, Krams Ret al., 2014, An In situ electroporation and flow device for mechanotransduction studies, Microarrays: Principles, Applications and Technologies, Pages: 49-67, ISBN: 9781629487137

© 2014 by Nova Science Publishers, Inc. All rights reserved. The ability of cells to receive and respond to chemical and physical signals from beyond the plasma membrane is fundamental to life. Sensing and processing of stimuli (such as hormones, mechanical factor s, light, heat, neurotransmitters, nutrients, odorants, etc.) is mediated by signal-transduction pathways, molecular circuits that detect, amplify, and integrate diverse external signals to govern functions such as cellular survival, proliferation, differentiation, and apoptosis. These information-processing circuits that control intra- and inter- cellular activities at a molecular level are extremely widespread and complex. For example, approximately half of the 25 largest protein families encoded by the human genome, deal primarily with information processing. Characterizing signalling pathways would not only offer a better understanding of life at molecular level, but would also allow the treatment of diseases. To characterize these extremely complex signalling pathways, high throughput systems are required that can add functions (gain-of-function) and delete functions (lossof- function) from primary cells by inserting cDNA and/or siRNA, respectively. Furthermore, in order to mimic physiologically relevant conditions, such as blood flow, cells need to be exposed to biomechanical stimulus. Here, we describe the design and experimental verification of a device for applying biomechanical stimulus in the form of fluid shear stress and electroporation transfection of primary adherent cells. To that end, primary cells are seeded in an incubation chamber on a coated conductive glass slide allowing in situ electroporation to be taken place. Electroporation with our device on hard-to-transfect endothelial cells yields an siRNA transfection efficiency of 70% while keeping cell viability above 95%. Next, the microfluidics functionality of the platform was proven by deleting the inherent flow alignment functio

BOOK CHAPTER

Kwak BR, Baeck M, Bochaton-Piallat M-L, Caligiuri G, Daemens MJAP, Davies PF, Hoefer IE, Holvoet P, Jo H, Krams R, Lehoux S, Monaco C, Steffens S, Virmani R, Weber C, Wentzel JJ, Evans PCet al., 2014, Biomechanical factors in atherosclerosis: mechanisms and clinical implications, EUROPEAN HEART JOURNAL, Vol: 35, Pages: 3013-+, ISSN: 0195-668X

JOURNAL ARTICLE

Maimari N, Broda K, Kakas A, Krams R, Russo Aet al., 2014, Symbolic Representation and Inference of Regulatory Network Structures, Logical Modeling of Biological Systems, Pages: 1-48, ISBN: 9781119005223

© ISTE Ltd 2014. All rights reserved. This chapter presents a general logic-based framework, called Abductive Regulatory Network Inference (ARNI), where it formalize the network extraction problem as an abductive inference problem. A general logical model is provided that integrates prior knowledge on molecular interactions and other information for capturing signal-propagation principles and compatibility with experimental data. Solutions to the abductive inference problem define signed-directed networks that explain how genes are affected during the experiments. Using in-silico datasets provided by the dialogue for reverse engineering assessments and methods (DREAM) consortium, the chapter demonstrates the improved predictive power and complexity of the inferred network topologies compared with those generated by other non-symbolic inference approaches. It also explores how the improved expressiveness together with the modularity and flexibility of the logic-based nature can support automated scientific discovery where the validity of hypothesized biological ideas can be examined and tested outside the laboratory.

BOOK CHAPTER

Mohri Z, Rowland E, Clarke LA, De Luca A, Krams R, Weinberg PDet al., 2014, ELEVATED ARTERIAL WALL PERMEABILITY MAY CAUSE VULNERABLE PLAQUE FORMATION IN MICE, Autumn Meeting of the British-Atherosclerosis-Society (BAS), Publisher: ELSEVIER IRELAND LTD, Pages: E6-E6, ISSN: 0021-9150

CONFERENCE PAPER

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