Imperial College London
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DrRobertKypta

Faculty of Medicine, Department of Surgery & Cancer

Senior Lecturer in Cancer Biology
 
 
 
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Contact

 

r.kypta Website

 
 
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Location

 

4007Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Murillo-Garzón:2018:10.1038/s41467-018-04042-w,
author = {Murillo-Garzón, V and Gorroño-Etxebarria, I and Åkerfelt, M and Puustinen, MC and Sistonen, L and Nees, M and Carton, J and Waxman, J and Kypta, RM},
doi = {10.1038/s41467-018-04042-w},
journal = {Nature Communications},
title = {Frizzled-8 integrates Wnt-11 and transforming growth factor-β signaling in prostate cancer},
url = {http://dx.doi.org/10.1038/s41467-018-04042-w},
volume = {9},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Wnt-11 promotes cancer cell migration and invasion independently of β-catenin but the receptors involved remain unknown. Here, we provide evidence that FZD8 is a major Wnt-11 receptor in prostate cancer that integrates Wnt-11 and TGF-β signals to promote EMT. FZD8 mRNA is upregulated in multiple prostate cancer datasets and in metastatic cancer cell lines in vitro and in vivo. Analysis of patient samples reveals increased levels of FZD8 in cancer, correlating with Wnt-11. FZD8 co-localizes and co-immunoprecipitates with Wnt-11 and potentiates Wnt-11 activation of ATF2-dependent transcription. FZD8 silencing reduces prostate cancer cell migration, invasion, three-dimensional (3D) organotypic cell growth, expression of EMT-related genes, and TGF-β/Smad-dependent signaling. Mechanistically, FZD8 forms a TGF-β-regulated complex with TGF-β receptors that is mediated by the extracellular domains of FZD8 and TGFBR1. Targeting FZD8 may therefore inhibit aberrant activation of both Wnt and TGF-β signals in prostate cancer.
AU  - Murillo-Garzón,V
AU  - Gorroño-Etxebarria,I
AU  - Åkerfelt,M
AU  - Puustinen,MC
AU  - Sistonen,L
AU  - Nees,M
AU  - Carton,J
AU  - Waxman,J
AU  - Kypta,RM
DO  - 10.1038/s41467-018-04042-w
PY  - 2018///
SN  - 2041-1723
TI  - Frizzled-8 integrates Wnt-11 and transforming growth factor-β signaling in prostate cancer
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-018-04042-w
UR  - http://hdl.handle.net/10044/1/57704
VL  - 9
ER  -
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