Imperial College London
Alternate

Dr. Rebekah E. T. Moore

Faculty of Engineering, Department of Earth Science & Engineering

Research Fellow
 
 
 
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Contact

 

r.moore13 Website

 
 
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Location

 

4.40/20Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Moore:2020:10.1038/s41438-020-0292-6,
author = {Moore, R and Ullah, I and de, Oliveira VH and Hammond, SJ and Strekopytov, S and Tibbett, M and Dunwell, JM and Rehkamper, M},
doi = {10.1038/s41438-020-0292-6},
journal = {Horticulture Research},
title = {Cadmium isotope fractionation reveals genetic variation in Cd uptake and translocation by Theobroma cacao and role of natural resistance-associated macrophage protein 5 and heavy metal ATPase-family transporters},
url = {http://dx.doi.org/10.1038/s41438-020-0292-6},
volume = {7},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In response to new European Union regulations, studies are underway to mitigate accumulation of toxic cadmium (Cd) in cacao (Theobroma cacao, Tc). This study advances such research with Cd isotope analyses of 19 genetically diverse cacao clones and yeast transformed to express cacao natural resistance-associated macrophage protein (NRAMP5) and heavy metal ATPases (HMAs). The plants were enriched in light Cd isotopes relative to the hydroponic solution with Δ114/110Cdtot-sol = −0.22 ± 0.08‰. Leaves show a systematic enrichment of isotopically heavy Cd relative to total plants, in accord with closed-system isotope fractionation of Δ114/110Cdseq-mob = −0.13‰, by sequestering isotopically light Cd in roots/stems and mobilisation of remaining Cd to leaves. The findings demonstrate that (i) transfer of Cd between roots and leaves is primarily unidirectional; (ii) different clones utilise similar pathways for Cd sequestration, which differ from those of other studied plants; (iii) clones differ in their efficiency of Cd sequestration. Transgenic yeast that expresses TcNRAMP5 (T. cacao natural resistance-associated macrophage gene) had isotopically lighter Cd than did cacao. This suggests that NRAMP5 transporters constitute an important pathway for uptake of Cd by cacao. Cd isotope signatures of transgenic yeast expressing HMA-family proteins suggest that they may contribute to Cd sequestration. The data are the first to record isotope fractionation induced by transporter proteins in vivo.
AU  - Moore,R
AU  - Ullah,I
AU  - de,Oliveira VH
AU  - Hammond,SJ
AU  - Strekopytov,S
AU  - Tibbett,M
AU  - Dunwell,JM
AU  - Rehkamper,M
DO  - 10.1038/s41438-020-0292-6
PY  - 2020///
SN  - 2052-7276
TI  - Cadmium isotope fractionation reveals genetic variation in Cd uptake and translocation by Theobroma cacao and role of natural resistance-associated macrophage protein 5 and heavy metal ATPase-family transporters
T2  - Horticulture Research
UR  - http://dx.doi.org/10.1038/s41438-020-0292-6
UR  - http://hdl.handle.net/10044/1/78962
VL  - 7
ER  -
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