Imperial College London

ProfessorRobinShattock

Faculty of MedicineDepartment of Infectious Disease

Chair in Mucosal Infection and Immunity
 
 
 
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Contact

 

+44 (0)20 7594 5206r.shattock

 
 
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Location

 

453Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cheeseman:2018:10.1089/hum.abc2018.075,
author = {Cheeseman, HM and Day, S and McFarlane, LR and Fleck, S and Miller, A and Cole, T and Sousa-Santos, N and Cope, A and Cizmeci, D and Tolazzi, M and Hwekwete, E and Hannaman, D and Kratochvil, S and McKay, PF and Chung, AW and Kent, SJ and Cook, A and Scarlatti, G and Abraham, S and Combadiere, B and McCormack, S and Lewis, DJ and Shattock, RJ},
doi = {10.1089/hum.abc2018.075},
journal = {Human Gene Therapy},
pages = {1011--1028},
title = {Combined Skin and Muscle DNA Priming Provides Enhanced Humoral Responses to a Human Immunodeficency Virus Type 1 Clade C Envelope Vaccine},
url = {http://dx.doi.org/10.1089/hum.abc2018.075},
volume = {29},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - © Copyright 2018, Mary Ann Liebert, Inc., publishers2018. Intradermal (i.d.) and intramuscular (i.m.) injections when administered with or without electroporation (EP) have the potential to tailor the immune response to DNA vaccination. This Phase I randomized controlled clinical trial in human immunodeficiency virus type 1-negative volunteers investigated whether the site and mode of DNA vaccination influences the quality of induced cellular and humoral immune responses following the DNA priming phase and subsequent protein boost with recombinant clade C CN54 gp140. A strategy of concurrent i.d. and i.m. DNA immunizations administered with or without EP was adopted. Subtle differences were observed in the shaping of vaccine-induced virus-specific CD4+ and CD8+ T cell-mediated immune responses between groups receiving: i.d.EP+ i.m., i.d. + i.m.EP, and i.d.EP+ i.m.EPregimens. The DNA priming phase induced 100% seroconversion in all of the groups. A single, non-adjuvanted protein boost induced a rapid and profound increase in binding antibodies in all groups, with a trend for higher responses in i.d.EP+ i.m.EP. The magnitude of antigen-specific binding immunoglobulin G correlated with neutralization of closely matched clade C 93MW965 virus and Fc-dimer receptor binding (FcγRIIa and FcγRIIIa). These results offer new perspectives on the use of combined skin and muscle DNA immunization in priming humoral and cellular responses to recombinant protein.
AU - Cheeseman,HM
AU - Day,S
AU - McFarlane,LR
AU - Fleck,S
AU - Miller,A
AU - Cole,T
AU - Sousa-Santos,N
AU - Cope,A
AU - Cizmeci,D
AU - Tolazzi,M
AU - Hwekwete,E
AU - Hannaman,D
AU - Kratochvil,S
AU - McKay,PF
AU - Chung,AW
AU - Kent,SJ
AU - Cook,A
AU - Scarlatti,G
AU - Abraham,S
AU - Combadiere,B
AU - McCormack,S
AU - Lewis,DJ
AU - Shattock,RJ
DO - 10.1089/hum.abc2018.075
EP - 1028
PY - 2018///
SN - 1043-0342
SP - 1011
TI - Combined Skin and Muscle DNA Priming Provides Enhanced Humoral Responses to a Human Immunodeficency Virus Type 1 Clade C Envelope Vaccine
T2 - Human Gene Therapy
UR - http://dx.doi.org/10.1089/hum.abc2018.075
UR - https://www.ncbi.nlm.nih.gov/pubmed/30027768
UR - http://hdl.handle.net/10044/1/61782
VL - 29
ER -