Imperial College London

ProfessorRobinShattock

Faculty of MedicineDepartment of Infectious Disease

Chair in Mucosal Infection and Immunity
 
 
 
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Contact

 

+44 (0)20 7594 5206r.shattock

 
 
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Location

 

453Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{McKay:2020:10.1038/s41467-020-17409-9,
author = {McKay, PF and Hu, K and Blakney, AK and Samnuan, K and Brown, JC and Penn, R and Zhou, J and Bouton, CR and Rogers, P and Polra, K and Lin, PJC and Barbosa, C and Tam, YK and Barclay, WS and Shattock, RJ},
doi = {10.1038/s41467-020-17409-9},
journal = {Nature Communications},
title = {Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine candidate induces high neutralizing antibody titers in mice},
url = {http://dx.doi.org/10.1038/s41467-020-17409-9},
volume = {11},
year = {2020}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The spread of the SARS-CoV-2 into a global pandemic within a few months of onset motivates the development of a rapidly scalable vaccine. Here, we present a self-amplifying RNA encoding the SARS-CoV-2 spike protein encapsulated within a lipid nanoparticle (LNP) as a vaccine. We observe remarkably high and dose-dependent SARS-CoV-2 specific antibody titers in mouse sera, as well as robust neutralization of both a pseudo-virus and wild-type virus. Upon further characterization we find that the neutralization is proportional to the quantity of specific IgG and of higher magnitude than recovered COVID-19 patients. saRNA LNP immunizations induce a Th1-biased response in mice, and there is no antibody-dependent enhancement (ADE) observed. Finally, we observe high cellular responses, as characterized by IFN-γ production, upon re-stimulation with SARS-CoV-2 peptides. These data provide insight into the vaccine design and evaluation of immunogenicity to enable rapid translation to the clinic.
AU - McKay,PF
AU - Hu,K
AU - Blakney,AK
AU - Samnuan,K
AU - Brown,JC
AU - Penn,R
AU - Zhou,J
AU - Bouton,CR
AU - Rogers,P
AU - Polra,K
AU - Lin,PJC
AU - Barbosa,C
AU - Tam,YK
AU - Barclay,WS
AU - Shattock,RJ
DO - 10.1038/s41467-020-17409-9
PY - 2020///
SN - 2041-1723
TI - Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine candidate induces high neutralizing antibody titers in mice
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/s41467-020-17409-9
UR - https://www.ncbi.nlm.nih.gov/pubmed/32647131
UR - http://hdl.handle.net/10044/1/80898
VL - 11
ER -