Imperial College London

ProfessorRobinShattock

Faculty of MedicineDepartment of Infectious Disease

Chair in Mucosal Infection and Immunity
 
 
 
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Contact

 

+44 (0)20 7594 5206r.shattock

 
 
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Location

 

453Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Fairhurst:2007:10.1021/la063043n,
author = {Fairhurst, D and Rowell, RL and Monahan, IM and Key, S and Stieh, D and McNeil-Watson, F and Morfesis, A and Mitchnick, M and Shattock, RA},
doi = {10.1021/la063043n},
journal = {Langmuir},
pages = {2680--2687},
title = {Microbicides for HIV/AIDS. 2. Electrophoretic fingerprinting of CD4+ T-cell model systems.},
url = {http://dx.doi.org/10.1021/la063043n},
volume = {23},
year = {2007}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - New measurements of the dependence of the surface charge on the pH and electrolyte concentration for three living human white blood cell lines that are the principal targets of the HIV-1 virus are reported. Comparison of the electrophoretic fingerprint (EF) pattern, especially the line of zero mobility, with that of reference colloids establishes the separate individual identities and shows that all three exhibit a zwitterionic surface. With the EF results as a guide, preliminary biological infectivity measurements showed that small polyvalent cations modulate the negative charge on the T-cell surface in a way that strongly affects the infection kinetics. H9 cells were exposed to an infectious virus (X4), and the data showed that HIV interaction with target cells is enhanced by physiological fluids. The nondestructive methodology described is generally applicable to characterization of the surface charge and determination of the colloidal stability of any aqueous charged colloidal system without reference to any model of the double layer.
AU - Fairhurst,D
AU - Rowell,RL
AU - Monahan,IM
AU - Key,S
AU - Stieh,D
AU - McNeil-Watson,F
AU - Morfesis,A
AU - Mitchnick,M
AU - Shattock,RA
DO - 10.1021/la063043n
EP - 2687
PY - 2007///
SN - 0743-7463
SP - 2680
TI - Microbicides for HIV/AIDS. 2. Electrophoretic fingerprinting of CD4+ T-cell model systems.
T2 - Langmuir
UR - http://dx.doi.org/10.1021/la063043n
UR - https://www.ncbi.nlm.nih.gov/pubmed/17266342
VL - 23
ER -