171 results found
Ang DL, Harper BWJ, Cubo L, et al., 2016, Back Cover: Quadruplex DNA-Stabilising Dinuclear Platinum(II) Terpyridine Complexes with Flexible Linkers (Chem. Eur. J. 7/2016), Chemistry - A European Journal, Vol: 22, Pages: 2540-2540, ISSN: 0947-6539
Turek VA, Francescato Y, Cadinu P, et al., 2015, Self-Assembled Spherical Supercluster Metamaterials from Nanoscale Building Blocks, ACS Photonics, Vol: 3, Pages: 35-42, ISSN: 2330-4022
We report on a simple, universal and large scale self-assembly method for generation of spherical superclusters from nanoscopic building blocks. The fundamentals of this approach relies on the ultra-high pre-concentration of nanoparticles (NP) followed by either using emulsification strategies or alternatively multiphase microfluidic microdroplets. In both cases drying of the NP droplets yield highly spherical self-assembled superclusters with unique optical properties. We demonstrate that the behaviour of these spheres can be controlled by surface functionalization before and after the self-assembly process. These structures show unique plasmonic collective response both on the surface and within the supercluster in the visible and infrared regions. Furthermore, we demonstrate that these strong, tunable optical modes can be used towards ultra-sensitive, reproducible, surface-enhanced spectroscopies.
Mion G, Gianferrara T, Bergamo A, et al., 2015, Phototoxic Activity and DNA Interactions of Water-Soluble Porphyrins and Their Rhenium(I) Conjugates, CHEMMEDCHEM, Vol: 10, Pages: 1901-1914, ISSN: 1860-7179
Shivalingam A, Izquierdo MA, Le Marois A, et al., 2015, The interactions between a small molecule and G-quadruplexes are visualised by fluorescence lifetime imaging microscopy, Nature Communications, Vol: 6, ISSN: 2041-1723
Guanine-rich oligonucleotides can fold into quadruple-stranded helical structures known as G-quadruplexes. Mounting experimental evidence has gathered suggesting that these non-canonical nucleic acid structures form in vivo and play essential biological roles. However, to date, there are no small-molecule optical probes to image G-quadruplexes in live cells. Herein, we report the design and development of a small fluorescent molecule, which can be used as an optical probe for G-quadruplexes. We demonstrate that the fluorescence lifetime of this new probe changes considerably upon interaction with different nucleic acid topologies. Specifically, longer fluorescence lifetimes are observed in vitro for G-quadruplexes than for double- and single-stranded nucleic acids. Cellular studies confirm that this molecule is cell permeable, has low cytotoxicity and localizes primarily in the cell nucleus. Furthermore, using fluorescence lifetime imaging microscopy, live-cell imaging suggests that the probe can be used to study the interaction of small molecules with G-quadruplexes in vivo.
Mendoza C, Jansat S, Vilar R, et al., 2015, Clickable complexing agents: functional crown ethers for immobilisation onto polymers and magnetic nanoparticles, RSC ADVANCES, Vol: 5, Pages: 87352-87363, ISSN: 2046-2069
Moffat CD, Weiss DJ, Shivalingam A, et al., 2014, Molecular Recognition and Scavenging of Arsenate from Aqueous Solution Using Dimetallic Receptors, Chemistry-A European Journal, Vol: 20, Pages: 17168-17177, ISSN: 1521-3765
A series of copper(II), nickel(II) and zinc(II) dimetallic complexes were prepared and their affinities towards arsenate investigated. Indicator displacement assays (IDAs) were carried out to establish the complexes with best affinities towards arsenate. A di-zinc complex (3) was selected and its arsenate-binding abilities investigated by isothermal titration calorimetry (ITC). The X-ray crystal structure of this metallo-receptor bound to arsenate is also reported, which allowed us to establish the binding mode between 3 and this oxyanion. Immobilising 3 onto HypoGel resin yielded a novel adsorbent (Zn–HypoGel) with high affinity for arsenate. Adsorption of arsenate from competitive solutions and natural groundwater was greater than that of the commercially used iron oxide Bayoxide E33. Zn–HypoGel could be efficiently and simply regenerated by washing with sodium acetate solution.
Stafford VS, Suntharalingam K, Shivalingam A, et al., 2014, Syntheses of polypyridyl metal complexes and studies of their interaction with quadruplex DNA, Dalton Transactions, Vol: 44, Pages: 3686-3700, ISSN: 1477-9226
A series of mono- and bi-metallic metal complexes (with CuII, PtII and ZnII) with substituted polypyridylligands have been prepared and their binding affinities towards quadruplex (c-Myc and human telomeric)and duplex DNA (ds26 and calf thymus) determined using fluorescent indicator displacement (FID) assaysand UV/vis spectroscopic titrations. These studies have shown that the number of aromatic rings andnumber/position of cyclic amine substituents on the ligands, play an important role in defining the DNAbinding abilities of the resulting metal complexes. We also show that bi-metallic complexes preparedusing a novel terpyridine-cyclen ligand have higher affinity towards G-quadruplex DNA as compared totheir mono-metallic counterparts. Cytotoxicity assays were carried out for all the new complexes againstan osteosarcoma cancer cell line (U2OS) as well as a normal fibroblast cell line (GM05757). Several ofthese compounds displayed cytotoxicity similar to that of cisplatin.
Cilibrizzi A, Collins J, Woscholski R, et al., 2014, New vanadium complexes as optical probes to detect Cys sulfenic modifications in PTEN, 12th European Biological Inorganic Chemistry Conference (EuroBIC), Publisher: Springer Verlag (Germany), Pages: S873-S873, ISSN: 1432-1327
Stafford V, Shivalingam A, Suntharalingam K, et al., 2014, Interaction of metal complexes with G-quadruplex DNA and their effects on gene expression, 12th European Biological Inorganic Chemistry Conference (EuroBIC), Publisher: SPRINGER, Pages: S740-S740, ISSN: 0949-8257
Zhou W, Vilar R, Ying L, et al., 2014, Transcriptional regulatory roles of G-quadruplex DNA in promoters of genes involved in beta-adrenergic signaling pathway, CARDIOVASCULAR RESEARCH, Vol: 103, ISSN: 0008-6363
Sala R, Quang-De N, Patel CBK, et al., 2014, Phosphorylation Status of Thymidine Kinase 1 Following Antiproliferative Drug Treatment Mediates 3 '-Deoxy-3 '-[F-18]-Fluorothymidine Cellular Retention, PLOS ONE, Vol: 9, ISSN: 1932-6203
Ghosh S, Mendoza O, Cubo L, et al., 2014, Assembly of Palladium(II) and Platinum(II) Metallo-Rectangles with a Guanosine-Substituted Terpyridine and Study of Their Interactions with Quadruplex DNA, CHEMISTRY-A EUROPEAN JOURNAL, Vol: 20, Pages: 4772-4779, ISSN: 0947-6539
D'Arcy M, Bullough F, Moffat C, et al., 2014, Adsorption of Oxy-Anions in the Teaching Laboratory: An Experiment To Study a Fundamental Environmental Engineering Problem, JOURNAL OF CHEMICAL EDUCATION, Vol: 91, Pages: 505-510, ISSN: 0021-9584
Leczkowska A, Vilar R, 2014, Targeting quadruplex nucleic acids with metal complexes, 16th International Conference on Biological Inorganic Chemistry (ICBIC), Publisher: SPRINGER, Pages: S378-S378, ISSN: 0949-8257
Pujol A, Vilar R, 2014, Multimetallic and Bifunctional MRI-probes for imaging apoptotic cells., 16th International Conference on Biological Inorganic Chemistry (ICBIC), Publisher: SPRINGER, Pages: S620-S620, ISSN: 0949-8257
Ali SMH, Yan Y-K, Lee PPF, et al., 2014, Copper(II) complexes of substituted salicylaldehyde dibenzyl semicarbazones: synthesis, cytotoxicity and interaction with quadruplex DNA, DALTON TRANSACTIONS, Vol: 43, Pages: 1449-1459, ISSN: 1477-9226
Karim NHA, Mendoza O, Shivalingam A, et al., 2014, Salphen metal complexes as tunable G-quadruplex binders and optical probes, RSC ADVANCES, Vol: 4, Pages: 3355-3363, ISSN: 2046-2069
Łȩczkowska A, Vilar R, 2013, Interaction of metal complexes with nucleic acids, Annual Reports on the Progress of Chemistry - Section A, Vol: 109, Pages: 299-316, ISSN: 0260-1818
This chapter reviews the literature reported during 2012 in the field of interaction of metal complexes with nucleic acids, also covering complex formation between transition metal ions and coordinating moieties within nucleic acids. As for the previous year, we have divided the chapter into three sections. The first focuses on the interaction of metal centres with DNA and is organised according to mode of interaction. The second section presents studies of metal complexes and metal ions that interact with RNA. In the last section we discuss examples where transition metals occupy coordination sites covalently incorporated within nucleic acids strands. © 2013 The Royal Society of Chemistry.
von Grebe P, Suntharalingam K, Vilar R, et al., 2013, A Conformationally Flexible Dinuclear Pt-II Complex with Differential Behavior of its Two States toward Quadruplex DNA, CHEMISTRY-A EUROPEAN JOURNAL, Vol: 19, Pages: 11429-11438, ISSN: 0947-6539
Whyte GF, Vilar R, Woscholski R, 2013, Molecular recognition with boronic acids-applications in chemical biology., J Chem Biol, Vol: 6, Pages: 161-174, ISSN: 1864-6158
Small molecules have long been used for the selective recognition of a wide range of analytes. The ability of these chemical receptors to recognise and bind to specific targets mimics certain biological processes (such as protein-substrate interactions) and has therefore attracted recent interest. Due to the abundance of biological molecules possessing polyhydroxy motifs, boronic acids-which form five-membered boronate esters with diols-have become increasingly popular in the synthesis of small chemical receptors. Their targets include biological materials and natural products including phosphatidylinositol bisphosphate, saccharides and polysaccharides, nucleic acids, metal ions and the neurotransmitter dopamine. This review will focus on the many ways in which small chemical receptors based on boronic acids have been used as biochemical tools for various purposes, including sensing and detection of analytes, interference in signalling pathways, enzyme inhibition and cell delivery systems. The most recent developments in each area will be highlighted.
Cowell S, Nguyen Q-D, Twyman F, et al., 2013, Towards multimodal molecular probes responsive to matrix metalloproteinases, JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, Vol: 56, Pages: S235-S235, ISSN: 0362-4803
Sala R, Quang-De N, Patel C, et al., 2013, Regulation of 18F-fluorothymidine uptake by thymidine kinase 1 protein phosphorylation., 104th Annual Meeting of the American-Association-for-Cancer-Research (AACR), Publisher: AMER ASSOC CANCER RESEARCH, ISSN: 0008-5472
Zhou W, Suntharalingam K, Brand NJ, et al., 2013, Possible Regulatory Roles of Promoter G-Quadruplexes in Cardiac Function-Related Genes - Human TnIc as a Model, PLOS One, Vol: 8, ISSN: 1932-6203
G-quadruplexes (G4s) are four-stranded DNA secondary structures, which are involved in a diverse range of biological processes. Although the anti-cancer potential of G4s in oncogene promoters has been thoroughly investigated, the functions of promoter G4s in non-cancer-related genes are not well understood. We have explored the possible regulatory roles of promoter G4s in cardiac function-related genes using both computational and a wide range of experimental approaches. According to our bioinformatics results, it was found that potential G4-forming sequences are particularly enriched in the transcription regulatory regions (TRRs) of cardiac function-related genes. Subsequently, the promoter of human cardiac troponin I (TnIc) was chosen as a model, and G4s found in this region were subjected to biophysical characterisations. The chromosome 19 specific minisatellite G4 sequence (MNSG4) and near transcription start site (TSS) G4 sequence (−80 G4) adopt anti-parallel and parallel structures respectively in 100 mM KCl, with stabilities comparable to those of oncogene G4s. It was also found that TnIc G4s act cooperatively as enhancers in gene expression regulation in HEK293 cells, when stabilised by a synthetic G4-binding ligand. This study provides the first evidence of the biological significance of promoter G4s in cardiac function-related genes. The feasibility of using a single ligand to target multiple G4s in a particular gene has also been discussed.
Suntharalingam K, Mendoza O, Duarte AA, et al., 2013, A platinum complex that binds non-covalently to DNA and induces cell death via a different mechanism than cisplatin, METALLOMICS, Vol: 5, Pages: 514-523, ISSN: 1756-5901
Ghosh S, Silber GT, White AJP, et al., 2013, Synthesis of a self-assembled copper(II) metallo-rectangle with a guanosine-substituted terpyridine, DALTON TRANSACTIONS, Vol: 42, Pages: 13813-13816, ISSN: 1477-9226
Jimeno C, Christmann U, Escudero-Adan EC, et al., 2012, Studies on the Amination of Aryl Chlorides with a Monoligated Palladium Catalyst: Kinetic Evidence for a Cooperative Mechanism, CHEMISTRY-A EUROPEAN JOURNAL, Vol: 18, Pages: 16510-16516, ISSN: 0947-6539
Suntharalingam K, Leczkowska A, Furrer MA, et al., 2012, A Cyclometallated Platinum Complex as a Selective Optical Switch for Quadruplex DNA, CHEMISTRY-A EUROPEAN JOURNAL, Vol: 18, Pages: 16277-16282, ISSN: 0947-6539
Łeczkowska A, Vilar R, 2012, Interaction of metal complexes with nucleic acids, Annual Reports on the Progress of Chemistry - Section A, Vol: 108, Pages: 330-349, ISSN: 0260-1818
This chapter reviews the literature reported during 2011 in the field of interaction of metal complexes with nucleic acids, also covering complex formation between transition metal ions and coordinating moieties within nucleic acids. As for the previous year, we have divided the chapter into three sections. The first focuses on the interaction of metal centres with DNA and is organised according to mode of interaction. The second section presents studies of metal complexes and metal ions that interact with RNA. In the last section we discuss examples where transition metals occupy coordination sites covalently incorporated within nucleic acids strands. © 2012 The Royal Society of Chemistry.
Suntharalingam K, Hunt DJ, Duarte AA, et al., 2012, A Tri-copper(II) Complex Displaying DNA-Cleaving Properties and Antiproliferative Activity against Cancer Cells, CHEMISTRY-A EUROPEAN JOURNAL, Vol: 18, Pages: 15133-15141, ISSN: 0947-6539
Tate CW, Gee AD, Vilar R, et al., 2012, Reversible carbon monoxide binding at copper(I) P-S-X (X=N, O) coordination polymers, JOURNAL OF ORGANOMETALLIC CHEMISTRY, Vol: 715, Pages: 39-42, ISSN: 0022-328X
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