Imperial College London

Prof. Ramon Vilar

Faculty of Natural SciencesDepartment of Chemistry

Professor of Medicinal Inorganic Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 1967r.vilar Website

 
 
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Location

 

301HMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Łęczkowska:2018:10.1002/chem.201802248,
author = {czkowska, A and Gonzalez-Garcia, J and Perez-Arnaiz, C and Garcia, B and White, AJP and Vilar, R},
doi = {10.1002/chem.201802248},
journal = {Chemistry - A European Journal},
pages = {11785--11794},
title = {Binding studies of metal-salphen and metal-bipyridine complexes towards G-quadruplex DNA},
url = {http://dx.doi.org/10.1002/chem.201802248},
volume = {24},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The proposed in vivo formation of G-quadruplex DNA (G4 DNA) in promoter regions of oncogenes and in telomeres has prompted the development of small molecules with high affinity and selectivity for these structures. Herein we report the synthesis of a new di-substituted bipyridine ligand and the corresponding complexes with Ni2+ and VO2+ . Both these new complexes have been characterized spectroscopically and by X-ray crystallography. Detailed DNA binding studies of these two complexes, together with three previously reported metal salphen complexes, are presented. Using FRET melting assays, the binding affinity and selectivity of the five metal complexes against six different G4 DNA structures as well as a duplex DNA have been determined. In addition, we present detailed ITC and UV/Vis studies to characterize the interaction of the complexes with human telomeric G4 DNA. Finally, we show via a polymerase stop assay that these complexes are able to stabilize a G4 DNA structure (from the c-Myc oncogene promoter) and halt the activity of Taq polymerase.
AU - czkowska,A
AU - Gonzalez-Garcia,J
AU - Perez-Arnaiz,C
AU - Garcia,B
AU - White,AJP
AU - Vilar,R
DO - 10.1002/chem.201802248
EP - 11794
PY - 2018///
SN - 0947-6539
SP - 11785
TI - Binding studies of metal-salphen and metal-bipyridine complexes towards G-quadruplex DNA
T2 - Chemistry - A European Journal
UR - http://dx.doi.org/10.1002/chem.201802248
UR - https://www.ncbi.nlm.nih.gov/pubmed/29897148
UR - http://hdl.handle.net/10044/1/61457
VL - 24
ER -